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[目的]研究姜黄素对紫外线致NIH3T3细胞损伤的保护作用。[方法]采用四甲基偶氮唑蓝比色法(MTT试验)和激光扫描共聚焦显微镜技术研究不同浓度(1、5、10μmol/L)的姜黄素对紫外线致细胞损伤的保护作用。[结果]与紫外线照射组(UVR组)相比较,姜黄素预处理(姜黄素+UVR)组可以提高紫外线辐照细胞的存活率(P<0.05),减少细胞内活性氧的堆积(P<0.05),减少细胞线粒体膜电位的下降(P<0.05)。[结论]姜黄素对紫外线致NIH3T3细胞损伤有保护作用,其作用机制可能与清除细胞内的活性氧,维持细胞的线粒体膜电位有关。
[Objective] The research aimed to study the protective effect of curcumin on NIH3T3 cell damage induced by ultraviolet light. [Method] The protective effects of curcumin at different concentrations (1, 5, 10μmol / L) on UV-induced cell injury were studied by MTT assay and laser scanning confocal microscopy. [Results] Curcumin pretreatment (curcumin + UVR) group could increase the survival rate of UV-irradiated cells (P <0.05) and decrease the accumulation of reactive oxygen species (ROS) in UV- 0.05), decreased mitochondrial membrane potential (P <0.05). [Conclusion] Curcumin has a protective effect on NIH3T3 cell injury induced by ultraviolet light. Its mechanism may be related to the elimination of reactive oxygen species and the maintenance of mitochondrial membrane potential.