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目的:探讨肺癌组织及其外周血浆、支气管肺泡灌洗液(BALF)中14-3-3σ基因启动子异常基因化状况及其在肺癌诊断中的价值。方法:用甲基化特异性PCR方法对肺癌组织、肺正常组织及相应血浆、BALF进行14-3-3σ基因启动子区CpG岛甲基化状态。结果:45例肺癌组织中,14-3-3σ基因启动子异常甲基化率为51.11%(23/45),相应血浆中14-3-3σ的甲基化检出率为31.11%(14/45),BALF检出率为42.22%(19/45);而正常组织中的14-3-3σ启动子甲基化率为17.39(4/23)(χ2=7.229,P<0.01),正常对照血浆未检出甲基化,非肺癌患者BALF中甲基化检出率为2.22%(1/45);血浆、BALF中甲基化改变与肿瘤组织甲基化状况显著相关(P<0.01);但与患者年龄、性别、肿瘤大小差异无统计学意义(P>0.05),与肿瘤病理分类相关(SCLC、NSCLCχ2=5.156,P>0.01)。结论:血浆、BALF中14-3-3σ基因异常甲基化改变的检测在肺癌的特异诊断等方面有一定的应用价值。
Objective: To investigate the abnormal gene expression of 14-3-3σ gene promoter in lung cancer tissues, peripheral blood plasma and bronchoalveolar lavage fluid (BALF) and its value in the diagnosis of lung cancer. Methods: Methylation status of CpG island in promoter region of 14-3-3σ gene was detected by methylation-specific PCR in lung cancer tissues, normal lung tissues and corresponding plasma and BALF. Results: The abnormal methylation rate of 14-3-3σ gene promoter was 51.11% (23/45) in 45 lung cancer tissues, and the methylation rate of 14-3-3σ in the corresponding plasma was 31.11% (14 / 45). The detection rate of BALF was 42.22% (19/45), while the methylation rate of 14-3-3σ promoter in normal tissues was 17.39 (4/23) (χ2 = 7.229, P <0.01) The methylation level in non-lung cancer patients with normal controls was 2.22% (1/45), while the methylation status in plasma and BALF was significantly associated with tumor methylation (P < 0.01). However, there was no significant difference with age, sex and tumor size (P> 0.05), which was correlated with tumor pathological classification (SCLC, NSCLCχ2 = 5.156, P> 0.01). Conclusion: The detection of abnormal methylation of 14-3-3σ gene in plasma and BALF has certain value in the diagnosis of lung cancer.