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目的:研究卵巢癌病人基因组不稳定和转化生长因子β受体Ⅱ基因(TβR?Ⅱ)突变与其发生关系.方法:应用聚合酶链反应技术和单链构象多态性分析方法,分析D2S123、MSE5和TβR?Ⅱ三个位点微卫星变化,并检测TβR?Ⅱ突变.结果:15例卵巢癌中有3例表现微卫星不稳定,有2例检出TβR?Ⅱ突变.结论:部分卵巢癌病人基因组存在微卫星不稳定,与其发生有关.TβR?Ⅱ基因突变,使细胞失去对转化生长因子β的反应而发生肿瘤,可能为卵巢癌发生的分子机理之一“,”Objective:To study the instabilities of microsatellite DNA and mutations of transforming growth factor β receptor Ⅱ (TβR-Ⅱ) in ovarian cancers. Methods:Microsatellite D2S123、 Mse5 and TβR-Ⅱ were analysed by polymerase chain reaction (PCR). Mutations of TβR-Ⅱ were detected by single strand conformation polymorphism (PCR-SSCP). Results:20%(3/15) of patients showed microsatellite instabilities (MIN). Somatic mutations of TβR-Ⅱ were found in 2 of 15 patients with ovarian cancer. Conclusion: MIN exist in some pa...