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使用盐酸硫胺素农度为1.3ppm的MS培养基用试管培养茶树茎和叶的切片,在NAA浓度为10~(-7), 10~(-6),10~(-5), 10~(-4)M,激动素浓度为0、10~(-7)、 10~(-6)、10~(-5) 、10~(-4)M中,从茎切片中诱导出了愈伤组织。而叶切片,在NAA农度为10~(-5)到10~(-4)M间,激动素浓度为0、10~(-7), 10~(-6)、10~(-5), 10~(-4)M中,诱导出了愈伤组织。茎切片培养根的分化,是在NAA浓度为10~(-5)或10~(-4)M,激动素浓度为0 ,10~(-7)、10~(-6)M中,NAA浓度为l0~(-5)M,激动素浓度为0、10~(-7), 10~(-6)、10~(-5), 10~(-4)M中所发生的。而叶片块养根的分化发生在NAA浓度为10~(-4)M,激动素浓度0、10~(-7), 10~(-6)、10~(-5)M中。这两种切片培养的结果显示出作为植物激素,根的分化条件的范围比愈伤组织诱导条件的范围要狭小,且后者包含着前者的条件范围。而且与愈伤组织诱导相比,根的分化发生在NAA的浓度比激动素的浓度高的条件中,从愈伤组织中分化根不需要加激动素,在培养基中使爪10~(-4)M浓度就可以了。
The stems and leaves of tea plants were cultured in vitro with MS medium containing thiamine hydrochloride at a concentration of 1.3ppm. The stems and leaves of tea plants were cultured in NAA medium containing 10 ~ (-7), 10 ~ (-6), 10 ~ (-5), 10 ~ (-4) M, the concentration of kinetin was 0,10 ~ (-7), 10 ~ (-6), 10 ~ (-5) and 10 ~ (-4) M, Callus. However, in the leaf slice, NAA had a range of 10 ~ (-5) to 10 ~ (-4) M and kinetin concentrations of 0, 10 ~ (-7), 10 ~ (-6), 10 ~ ), 10 ~ (-4) M, the callus was induced. The root differentiation of stem section was in NAA concentration 10 ~ (-5) or 10 ~ (-4) M, kinetin concentration 0, 10 ~ (-7), 10 ~ (-6) M, NAA Concentration of l0 ~ (-5) M, kinetin concentration of 0,10 ~ (-7), 10 ~ (-6), 10 ~ (-5), 10 ~ (-4) M occurred. The rooting differentiation of leaf segments occurred in NAA concentration of 10 ~ (-4) M, kinetin concentrations of 0, 10 ~ (-7), 10 ~ (-6), 10 ~ (-5) M. The results of these two sections showed that the range of differentiation conditions of roots as plant hormones was narrower than that of callus induction conditions, and the latter included the former range of conditions. Moreover, compared with callus induction, root differentiation occurred at a higher concentration of NAA than that of kinetin. There was no need to add kinetin to differentiate roots from callus, 4) M concentration on it.