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目的探讨前列腺癌细胞中特异性标志物lncRNA PCA3的异常表达与前列腺癌细胞的异常增殖的关系,以及lncRNA PCA3与邻近的PRUNE2 mRNA间的关系。方法以前列腺癌标准细胞株LNCaP细胞为研究对象,针对lncRNA PCA3和PRUNE2 mRNA分别设计特异性shRNA序列,构建不同shRNA慢病毒转染LNCaP细胞。RT-PCR法检测前列腺癌细胞感染lncRNA PCA3慢病毒后的增殖情况,以及lncRNA PCA3和PRUNE2 mRNA的表达改变。结果慢病毒抑制lncRNA PCA3的表达后,能够检测到LNCaP细胞生长的减慢;相对于阴性对照组,在lncRNA PCA3受到抑制后,PRUNE2 mRNA的表达增加(P<0.05)。反之,当PRUNE2 mRNA的表达受到抑制,则lncRNA PCA3的表达增加(P<0.05)。结论 lncRNA PCA3是影响LNCaP细胞增殖的一个重要因素,其互补链上反向表达的PRUNE2基因是一个抑制lncRNA PCA3表达的抑癌基因,可能是一个未知的有意义的治疗靶点。
Objective To investigate the relationship between abnormal expression of prostate cancer cell lncRNA PCA3 and abnormal proliferation of prostate cancer cells and the relationship between lncRNA PCA3 and adjacent PRUNE2 mRNA. Methods LNCaP cells, a standard prostate cancer cell line, were used as research objects. Specific shRNA sequences were designed according to lncRNA PCA3 and PRUNE2 mRNA, and different shRNA lentivirus were transfected into LNCaP cells. Proliferation of prostate cancer cells infected with lncRNA PCA3 lentivirus and the expression of lncRNA PCA3 and PRUNE2 mRNA were detected by RT-PCR. Results After lentivirus inhibited the expression of lncRNA PCA3, the growth of LNCaP cells was slowed down. Compared with the negative control group, the expression of PRUNE2 mRNA increased after lncRNA PCA3 was inhibited (P <0.05). Conversely, when the expression of PRUNE2 mRNA was inhibited, the expression of lncRNA PCA3 was increased (P <0.05). Conclusions lncRNA PCA3 is an important factor that affects the proliferation of LNCaP cells. The PRUNE2 gene, whose expression is reversely expressed on the complementary strand, is a tumor suppressor gene that inhibits the expression of lncRNA PCA3, which may be an unknown therapeutic target.