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目的 探讨儿童特发性血小板减少性紫癜(ITP)自身免疫的病理机制,并为治疗提供理论依据。方法 对66例ITP患儿及38例健康儿童利用APAAP法检测外周血T细胞亚群,同时对66例ITP患儿及52例健康成人采用酶联免疫吸附竞争试验原理定量测定血小板相关抗体PAIgG含量。结果 所有病例CD3+、CD4+及CD4+/CD8+值下降,经统计学处理ITP组低于对照组(P<001或005);CD8+值高于对照组(P<001)。ITP组PAIgG的结果与对照组比较明显升高(P<001)。结论 ITP的发病机制不仅与B细胞功能异常有关,且与T淋巴细胞的表达及功能改变亦密切相关。
Objective To investigate the pathogenesis of idiopathic thrombocytopenic purpura (ITP) autoimmunity in children and to provide a theoretical basis for the treatment. Methods Totally 66 children with ITP and 38 healthy children were tested for peripheral blood T cell subsets by APAAP method. 66 patients with ITP and 52 healthy adults were enrolled in this study. Enzyme-linked immunosorbent assay (ELISA) was used to determine PAIgG . Results The values of CD3 +, CD4 + and CD4 + / CD8 + decreased in all cases. The ITP group was lower than the control group (P <001 or 005), and the CD8 + value was higher than that of the control group (P <001). Results of PAIgG in ITP group were significantly higher than those in control group (P <001). Conclusion The pathogenesis of ITP is not only related to the abnormal function of B cells, but also to the expression and function of T lymphocytes.