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1985—1987年对苏芸金杆菌防治印度谷螟的效果进行研究,用JMEM-1200W型透射电于显微镜观察,幼虫食入菌剂后1至2小时,中肠出现细胞松离,肠壁和底膜崩溃,肠内物质散入肠腔,16至24小时后,幼虫陆续变暗褐色而死亡。实验室内用菌悬液对幼虫进行生物测定,浓度为2.266×10~8个活芽孢子/毫升时,死亡率在32小时内达98.24％,随着处理浓度降底,幼虫死亡率下降。以粉剂在储粮表面层10—15厘米深处理杀虫效果最佳,持效期长,仓库条件下5个月后,平均每粒花生上仍有活芽孢3.13×10~3个。温度是影响该菌剂杀虫效果的重要因素,而湿度与之无关,温湿度之间也无互作。实验室内菌种转移接种16代以后杀虫毒力下降。
From 1985 to 1987, the effect of Bacillus thuringiensis on the control of Indian rice stem borer was studied. With JMEM-1200W transmission electron microscope, the larvae inoculated with the bacteria for 1 to 2 hours, After the film collapses, enteric substances are scattered into the intestine. After 16 to 24 hours, the larvae gradually dim brown and die. In the laboratory, the larvae were bioassayed by the bacterial suspension. When the concentration was 2.266 × 10 ~ 8 spores / ml, the mortality rate reached 98.24% within 32 hours. With the decrease of the treatment concentration, the larval mortality decreased. The powder with the surface layer of stored grain 10-15 cm depth of the best insecticidal effect, long duration, after 5 months of storage conditions, the average number of live spores per peanut 3.13 × 10 ~ 3. Temperature is an important factor affecting insecticidal effect of the fungicide, and humidity has nothing to do with the interaction between temperature and humidity no interaction. After 16 generations of inoculation in laboratory, insecticidal virulence decreased.