论文部分内容阅读
目的:在明确Ad-14-3-3σ对不同辐射抗拒鼻咽癌(nasopharygycarcinoma,NPC)细胞CNE-1和CNE-2治疗作用的基础上,检测Ad-14-3-3σ对CNE-1和CNE-2细胞中差异表达的微小RNA(microR-NA,miRNA)的影响,探索CNE-1和CNE-2细胞中miRNA差异表达与NPC放射敏感性差异的关系。方法:用Ad-14-3-3σ转染CNE-1和CNE-2细胞;采用Paraflo microfluidic microRNA芯片检测,用激光扫描器收集杂交的图像,经LOWESS滤器规范信号后分析数据差异,根据Targetscan3.1数据库资料(http://www.targetscan.org)探索Ad-14-3-3σ对CNE-1和CNE-2中的miRNA差异表达的影响,预测其差异表达与NPC放射敏感性差异的关系。结果:Ad-14-3-3σ处理细胞以后,CNE-1与CNE-2相比较,有37个microRNA有明显差异,CNE-1中有17个上调,20个下调。其中倍数变化在3倍以上且2者检测量数量差异达到1000以上的有6个:hsa-miR-152、hsa-miR-205、hsa-miR-203、hsa-miR-7、hsa-miR-636和hsa-miR-100。结论:Ad-14-3-3σ能够改变微小RNA(microRNA,miRNA)在CNE-1和CNE-2中的表达模式,缩小CNE-1和CNE-2之间miRNA的表达差异,而这些miRNA可能跟肿瘤的发生和放疗敏感性有关。
OBJECTIVE: To investigate the therapeutic effect of Ad-14-3-3σ on CNE-1 and CNE-2 cells treated with different doses of radioresistant nasopharyngeal carcinoma (NPC) cells, CNE-2 cells in order to explore the relationship between miRNA differential expression in CNE-1 and CNE-2 cells and NPC radiosensitivity. METHODS: CNE-1 and CNE-2 cells were transfected with Ad-14-3-3σ. Paraflo microfluidic microarray was used to detect the hybridization images. Laser scanning was used to collect the hybridization images. The data were analyzed by LOWESS filter and the data were analyzed according to Targetscan3. 1 Database (http://www.targetscan.org) To explore the effect of Ad-14-3-3σ on miRNA differential expression in CNE-1 and CNE-2 and to predict the relationship between differential expression and NPC radiosensitivity . RESULTS: After Ad-14-3-3σ treated cells, 37 microRNAs were significantly different between CNE-1 and CNE-2, with 17 up-regulated and 20 down-regulated in CNE-1. Among them, hsa-miR-152, hsa-miR-205, hsa-miR-203, hsa-miR-7 and hsa-miR- 636 and hsa-miR-100. Conclusions: Ad-14-3-3σ can change the expression pattern of microRNAs (miRNAs) in CNE-1 and CNE-2 and reduce the miRNA expression difference between CNE-1 and CNE-2, and these miRNAs may With the occurrence of cancer and radiosensitivity.