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目的研究醛化白及多糖(DBsGM)/羟丙基壳聚糖(HPCS)/纳米珍珠层粉(NNP)复合支架材料对大鼠下颌骨缺损愈合过程的成骨促进作用,并观察该材料的降解吸收情况。方法取32只雄性Wistar大鼠,于每只大鼠双侧下颌磨牙区作箱型骨缺损,根据处死时间(2、4、6、8周)随机分成4组,每组8只,在4组中实验侧植入DBsGM/HPCS/NNP复合支架材料,对照侧不植入任何材料。切片经苏木精-伊红染色,组织学测量评价骨缺损修复效果。结果两组新生骨面积百分比随愈合时间延长逐渐增加(P<0.05);相同愈合时间的实验侧新生骨面积优于对照侧(P<0.05)。植入材料随着时间逐渐降解,第6~8周,植入材料基本降解完全。结论 DBsGM/HPCS/NNP复合支架材料在大鼠下颌骨缺损中具有良好的促进成骨作用。
OBJECTIVE To study the osteogenesis promoting effect of DBsGM / HPCS / NNP composite scaffold on the healing of mandibular defects in rats and to observe the effect of DBSGM / Degradation and absorption. Methods Thirty-two male Wistar rats were used to make box-bone defects in the bilateral mandibular molars of each rat. The rats were randomly divided into 4 groups (2, 4, 6 and 8 weeks) DBsGM / HPCS / NNP composite scaffolds were implanted on the experimental side in the group, and no material was implanted on the control side. The sections were stained with hematoxylin-eosin, histological measurements were used to evaluate the effect of bone defect repair. Results The percentages of newborn bone area in two groups increased gradually with the prolongation of healing time (P <0.05). The area of newborn bone in the same healing time was better than that in the control side (P <0.05). Implant materials gradually degrade over time, the first 6 to 8 weeks, the basic degradation of implant material completely. Conclusion DBsGM / HPCS / NNP composite scaffolds can promote osteogenesis in rat mandibular defects.