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目的探讨氯化镉对小鼠胚胎神经干细胞(m NSC)细胞活性和迁移能力的影响。方法建立小鼠神经干细胞体外模型,以0、0.1、0.3、1.0、3.0和10.0μmol/L的氯化镉作用于小鼠胚胎神经干细胞24 h,噻唑蓝(MTT)比色法测定细胞活力;免疫荧光细胞化学法分析细胞迁移。结果 10.0μmol/L的Cd Cl2作用24 h后细胞存活率(70.08±6.21)%显著低于正常对照组(P<0.05);在设定剂量下,氯化镉作用神经干细胞24 h后,Aa/Ab和Dm/Db呈下降趋势,存在剂量依赖性(rs Aa/Ab=-0.90,rs Dm/Db=-0.90,P<0.05)。结论氯化镉对神经干细胞的迁移和活力均有影响,且在同一浓度和相同作用时间下对神经干细胞的迁移影响大于对细胞活力的影响。
Objective To investigate the effect of cadmium chloride (CdCl2) on the activity and migration of mouse embryonic neural stem cells (m NSCs). Methods Mouse neural stem cells were cultured in vitro and treated with cadmium chloride (0, 0.1, 0.3, 1.0, 3.0 and 10.0 μmol / L) for 24 h. The viability of mouse neural stem cells was determined by MTT assay. Immunofluorescence cytochemistry analysis of cell migration. Results The cell viability (70.08 ± 6.21)% after treated with 10.0 μmol / L CdCl 2 for 24 h was significantly lower than that of the normal control (P <0.05). After exposure to cadmium chloride for 24 h, the survival rate of Aa / Ab and Dm / Db showed a downward trend in a dose-dependent manner (rs Aa / Ab = -0.90, rs Dm / Db = -0.90, P <0.05). Conclusion Cadmium chloride has an effect on the migration and viability of neural stem cells, and affects the migration of neural stem cells more than that of the cells at the same concentration and time.