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NPR1(non-expresser of pathogenesis related genes 1)是水杨酸(salicilic acid,SA)介导的植物系统获得抗性(systemic aquired resistance,SAR)的关键调控因子,在水稻中过量表达拟南芥NPR1和水稻NH1/OsNPR1(NPR1homologue 1)(NPR1的同源物)可增强抗病性。水稻基因组中还有4个NPR1旁系同源物(paralog)。为了研究水稻NPR1旁系同源物3,NH3(NPR1 homologue 3),本文利用其自身启动子驱动NH3基因的表达,构建载体将其转入水稻,获得了10个T0代转基因植株。PCR检测证明NH3基因已成功转入水稻中。
NPR1 (non-expresser of pathogenesis related genes 1) is a key regulator of salicylic acid (SA) -mediated systemic aquired resistance (SAR), overexpressing Arabidopsis NPR1 And rice NH1 / OsNPR1 (NPR1 homologue 1) (homolog of NPR1) enhances disease resistance. There are also four NPR1 paralogs in the rice genome. In order to study the NPR1 homologue3 and NH3 (NPR1 homologue 3) in rice, we used their own promoters to drive the expression of NH3 gene. The constructed vector was transformed into rice and 10 T0 generation transgenic plants were obtained. PCR test proved that the NH3 gene has been successfully transferred into rice.