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目的探讨还原型谷胱甘肽(GSH)在预防糖尿病大鼠勃起功能障碍中的作用。方法通过腹腔注射链脲佐菌素65mg/kg建立糖尿病大鼠模型,然后随机分成DM组和DM+GSH组,DM+GSH组每天肌肉注射GSH200mg/kg。10周后观察大鼠勃起功能,并获取海绵体组织检测其谷胱甘肽、一氧化氮合酶(NOS)与丙二醛(MDA)水平,用TUNEL法检测细胞凋亡。结果成功建立糖尿病大鼠模型。与未注射GSH的DM组相比,DM+GSH组和正常对照组(C组)勃起功能更好,勃起率分别是20%,62.5%和100%。GSH水平DM+GSH组和C组明显比DM组高,其3组含量每克蛋白分别是(75.83±15.62)、(61.47±8.65)和(35.03±12.29)mg(P<0.05);NOS水平在DM+GSH组每毫克蛋白为(133.9±31.9)U,与正常对照组每毫克蛋白为(142.2±31.2)U相当,但较DM组每毫克蛋白为(58.4±18.9)U高(P<0.05);MDA含量在DM组每毫克蛋白为(3.71±0.62)nmol,明显高于正常对照组和DM+GSH组(P<0.05),这两组每毫克蛋白为(2.08±0.34)nmol和(2.44±0.28)nmol;细胞凋亡率在DM组、DM+GSH组和C组的分别是(22.6±3.6)%、(10.8±1.7)%和(7.2±2.1)%(P<0.05)。结论还原型谷胱甘肽对糖尿病大鼠阴茎组织有较好的抗氧化作用,能减少细胞凋亡,对延缓糖尿病性ED的发生有一定的作用。
Objective To investigate the role of reduced glutathione (GSH) in the prevention of erectile dysfunction in diabetic rats. Methods Diabetic rats were induced by intraperitoneal injection of streptozotocin (65mg / kg) and then randomly divided into DM group and DM + GSH group. Rats in DM + GSH group were intramuscularly injected with GSH 200mg / kg. Eighteen weeks later, the erectile function of the rats was observed. The cavernous tissues were collected to detect the levels of glutathione, nitric oxide synthase (NOS) and malondialdehyde (MDA). TUNEL method was used to detect the apoptosis. Results The diabetic rat model was successfully established. Compared with the DM group without GSH injection, the erectile function was better in DM + GSH group and normal control group (C group), with erectile rates of 20%, 62.5% and 100%, respectively. GSH levels in DM + GSH group and C group were significantly higher than those in DM group (75.83 ± 15.62), (61.47 ± 8.65) and (35.03 ± 12.29) mg (P <0.05), respectively. The levels of NOS In the DM + GSH group, the protein per milligram was (133.9 ± 31.9) U, which was equivalent to (142.2 ± 31.2) U per mg of protein in the normal control group and 58.4 ± 18.9 U per mg of protein in the DM group (P < 0.05). The content of MDA in DM group was (3.71 ± 0.62) nmol, which was significantly higher than that in normal control group and DM + GSH group (P <0.05) (2.44 ± 0.28) nmol respectively. The rates of apoptosis were (22.6 ± 3.6)%, (10.8 ± 1.7)% and (7.2 ± 2.1)% in DM group, DM + GSH group and C group (P < . Conclusion Reduced glutathione has a good anti-oxidative effect on the penile tissue of diabetic rats, which can reduce the apoptosis of cells and delay the development of diabetic ED.