论文部分内容阅读
目的:阐明血吸虫病肝纤维化时肝脏Ⅵ型前胶原m RNA表达和胶原含量的变化。方法:采用Northern核酸分子杂交和免疫组化技术。结果:小鼠感染后8 wk 时,肝内α1(Ⅵ)前胶原m RNA的表达量显著增加,10 w k时,其表达值达高峰,12～16 w k,略有下降,但直至20 wk 仍维持在较高水平。免疫组化染色显示,肝内Ⅵ型胶原在感染后8 wk 时出现于中央静脉周围和肝窦壁,10 w k 时,在肝组织内继续增多,12 w k 时虫卵肉芽肿内明显着色,16 w k 时,其含量达高峰,20 wk 时,广泛分布于汇管区、虫卵肉芽肿内及其周围,并形成致密的网络间隔。结论:血吸虫病肝纤维化形成过程中肝脏α1(Ⅵ)前胶原m RNA 表达和胶原含量均有显著增加,表明Ⅵ型胶原的检测对血吸虫病肝纤维化的评估可能有重要价值
Objective: To clarify the hepatic expression of type Ⅵ procollagen mRNA and collagen in schistosomiasis patients with liver fibrosis. Methods: Northern hybridization and immunohistochemistry were used. RESULTS: At 8 weeks post-infection, the mRNA expression of α1 (Ⅵ) procollagen in the liver increased significantly, reaching a peak at 10 wk with a slight decrease from 12 to 16 wk but up to 20 wk Maintain at a high level. Immunohistochemical staining showed that intrahepatic collagen type Ⅵ appeared around the central vein and the hepatic sinusoid wall at 8 weeks postinfection, and continued to increase in the liver tissue at 10 weeks post-infection. Significant staining was observed in the egg granuloma at 12 weeks wk, its content reached the peak, 20 wk, widely distributed in the portal area, the egg granuloma and its surroundings, and the formation of dense network interval. CONCLUSIONS: The expression of hepatic α1 (Ⅵ) procollagen m RNA and collagen content in schistosomiasis hepatic fibrosis are significantly increased, indicating that the detection of type Ⅵ collagen may have important value in the evaluation of schistosomiasis hepatic fibrosis