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目的通过研究胶体金法与气相色谱法在检测毒死蜱中的应用,证实胶体金试纸条在检测蔬菜中毒死蜱残留的可靠性。方法对比胶体金试纸半定量法和气相色谱定量法检测毒死蜱的结果。结果胶体金试纸条检测阴性样品得出的结果与气相色谱的结果符合率为100%,添加的阳性样品符合率也达到100%。添加的界限值样品的回收率达到96%。结论与气相色谱法相比较,胶体金试纸检测毒死蜱操作简便、观察直观、快速、省时,其特异性、敏感性较高,可作为蔬菜采摘前毒死蜱农药残留自我检测的手段。并可以此为基础建立一个不同于以前的毒死蜱监管模式。
Objective To study the application of colloidal gold method and gas chromatography in the detection of chlorpyrifos and confirm the reliability of colloidal gold test strip for detecting chlorpyrifos residues in vegetables. Methods Colloidal gold test strip semi-quantitative and gas chromatography quantitative detection of chlorpyrifos results. Results The result of colloidal gold test strip test for negative samples was 100% accordant with that of gas chromatogram and the coincidence rate of positive samples added was 100%. The cut-off value of the sample was recovered to 96%. Conclusion Compared with gas chromatography, the detection of chlorpyrifos by colloidal gold test strip is simple, intuitive, rapid and time-saving. Its specificity and sensitivity are high and can be used as a means of self-detection of pesticide residues of chlorpyrifos in vegetables. And based on this, a regulatory model different from previous chlorpyrifos can be established.