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本试验应用Co~(60)-γ射线及紫外线诱变抗生素T_(21)产生菌,力求探讨其对菌的致死作用和杀虫活力的影响。 (一)材料和方法 1.出发菌株:浅黄链霉素韶关变种Streptomy-ces flaveolus var.Shaoguanensis n.var.21-16。 2.培养基:①斜面和分离培养基。KNO_30.1％、K_2HPO_40.05％、MgSO_40.05％、NaC10、05％、FeSO_40.001％、可溶性淀粉2％、琼脂2％、pH7.2～7.4。120℃灭菌30分钟。②摇瓶发酵培养基。淀粉3％、葡萄糖1％、蚕蛹粉2％、酵母粉0.5％、MgSO_40.05％、CaCO_30.06％、pH7.0、120℃灭菌
In this experiment, Co ~ (60) -γ ray and ultraviolet mutagenesis were used to mutate the antibiotic T_ (21) -producing bacterium. The aim of this study was to investigate the effects on the lethality and insecticidal activity of the bacterium. (A) Materials and methods 1. Starting strain: Streptomyces cv. Flaveolus var. Shaoguanensis n.var.21-16. 2. Medium: ① slant and separation medium. KNO_30.1%, K_2HPO_40.05%, MgSO_40.05%, NaC10,05%, FeSO_40.001%, soluble starch 2%, agar 2%, pH7.2 ~ 7.4.120 ℃ for 30 minutes. ② shake flask fermentation medium. Starch 3%, glucose 1%, silkworm pupa powder 2%, yeast powder 0.5%, MgSO 40.05%, CaCO 3 0.06%, pH 7.0, sterilized at 120 ° C