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目的:探讨化瘀行水验方抗大鼠肾间质性纤维化的作用。方法:将大鼠随机分为单侧输尿管结扎造模组(UUO)和假手术对照组。单侧输尿管结扎造模后大鼠进一步被随机分为模型组、化瘀行水验方治疗组(中药组)和卡托普利组。分别于用药第7天、14天、21天和28天观察梗阻侧肾间质的胶原纤维、金属蛋白酶-9(MMP-9)和基质金属蛋白酶抑制剂-1(TIMP-1)的表达情况。结果:UUO组各时间点与假手术组相比:肾间质胶原纤维、MMP-9和TIMP-1的表达增加有显著统计学差异(P<0.05或P<0.01)。中药组和卡托普利组与模型组相比,用药7天时,可减少肾间质胶原纤维的病理性沉积和TIMP-1的表达(P<0.05),但MMP-9表达差异无明显的统计学意义;用药14天、21天和28天时,可进一步显著减少肾间质胶原纤维的病理性沉积和TIMP-1的表达(P<0.01),但未降至假手术组水平(P<0.01),可增加MMP-9的表达(P<0.05)。结论:化瘀行水验方治疗后,可减少单侧输尿管结扎大鼠肾间质胶原纤维的病理性沉积和TIMP-1的表达,增加MMP-9的表达,对拮抗肾间质纤维化有一定的作用。
Objective: To investigate the effect of Huatan Xingshuifang on anti-renal interstitial fibrosis in rats. METHODS: Rats were randomly divided into unilateral ureteral ligation model group (UUO) and sham-operated control group. After unilateral ureteral ligation, the rats were further randomly divided into model group, Huayu Xingshuifang treatment group (TCM group) and captopril group. The expression of collagen fibers, metalloproteinase-9 (MMP-9), and matrix metalloproteinase inhibitor-1 (TIMP-1) in the renal interstitium of the obstructed side were observed on the 7th, 14th, 21st, and 28th days respectively. . RESULTS: Compared with the sham group at each time point in the UUO group, the expression of collagen fibrils, MMP-9, and TIMP-1 increased significantly (P<0.05 or P<0.01). Compared with the model group, Chinese medicine group and captopril group can reduce the pathological deposition of renal interstitial collagen fibers and the expression of TIMP-1 (P<0.05), but there is no significant difference in the expression of MMP-9. Statistical significance; On the 14th, 21st and 28th days of treatment, the pathological deposition of renal interstitial collagen fibers and the expression of TIMP-1 were significantly reduced (P<0.01), but not to the sham group level (P< 0.01) increased the expression of MMP-9 (P<0.05). Conclusion: After treated with Huatanxingmi recipe, the pathological deposition of collagen fibers and the expression of TIMP-1 in unilateral ureteral obstruction rats can be reduced, and the expression of MMP-9 can be increased. It is certain to antagonize renal interstitial fibrosis. The role.