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目的探索TGF-β、全反式维甲酸及IL-2在大鼠初始T细胞向Foxp3+T细胞分化中的作用。方法分选Lewis大鼠脾脏CD4+CD25-初始T细胞,在抗CD3和CD28抗体激活下,分别用TGF-β、IL-2及全反式维甲酸诱导,通过流式细胞仪检测细胞表面CD25及胞内Foxp3的表达。并提取细胞mRNA通过逆转录PCR检测Treg功能相关基因的表达。结果 TGF-β在体外能诱导大鼠CD4+CD25-初始T细胞分化为CD4+CD25+Foxp3+T细胞,而IL-2或全反式维甲酸的单独作用不能诱导Foxp3表达。IL-2与全反式维甲酸能增强TGF-β诱导Foxp3表达的作用,在TGF-β、IL-2及全反式维甲酸的共同作用下,诱导的Foxp3+T细胞比例提高。TGF-β诱导的Foxp3+T细胞较高表达IL-10、CTLA-4等调节性T细胞功能相关基因。结论 TGF-β在体外能诱导大鼠CD4+CD25-初始T细胞分化为CD4+CD25+Foxp3+T细胞,IL-2与全反式维甲酸能增强这一作用。
Objective To explore the role of TGF-β, all-trans retinoic acid and IL-2 in the differentiation of rat primary T cells into Foxp3 + T cells. Methods The splenic CD4 + CD25- naive T cells from Lewis rats were induced by TGF-β, IL-2 and all-trans retinoic acid activated by anti-CD3 and CD28 antibodies. The cell surface CD25 And intracellular Foxp3 expression. And extract cell mRNA reverse transcription PCR detection of Treg function-related gene expression. Results TGF-β induced CD4 + CD25 + T cells to differentiate into CD4 + CD25 + Foxp3 + T cells in vitro, whereas IL-2 or all-trans retinoic acid alone did not induce Foxp3 expression. IL-2 and all-trans retinoic acid can enhance the expression of Foxp3 induced by TGF-β. The proportion of Foxp3 + T cells induced by TGF-β, IL-2 and all-trans retinoic acid is increased. TGF-β-induced Foxp3 + T cells express higher levels of IL-10, CTLA-4 and other regulatory T cell function-related genes. Conclusion TGF-β can induce the differentiation of CD4 + CD25- naive T cells into CD4 + CD25 + Foxp3 + T cells in vitro. IL-2 and all-trans retinoic acid can enhance this effect.