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目的:对比研究4种常用的检测IgG型血型抗体方法,确定不同方法的差异。方法:分别用凝聚胺法、试管间接抗球蛋白法(IAT)、LISS-凝胶卡法和LISS-试管间接抗球蛋白法(LISS-IAT)检测倍比稀释的抗-D,从临床配血不相合样本中检出的IgG抗-E、抗-Lea、抗-Jka和87例有输血史的患者样本。结果:对倍比稀释的抗-D,LISS-IAT法的检测灵敏度最高,凝聚胺法最低;对于弱的IgG抗体,凝聚胺法会漏检,IAT也存在漏检的可能,而LISS-凝胶卡法和LISS-IAT法均能检出弱的抗体;87例有输血史患者的抗筛结果显示,LISS-凝胶卡法和LISS-IAT法的检出不规则抗体例数都达到10例,高于其他2种方法。结论:免疫血清学实验室应采用更为敏感的实验技术检测血型抗体,如LISS-凝胶卡法或LISS-IAT法,以保障临床输血安全。
OBJECTIVE: To compare and analyze four commonly used methods of detecting IgG type blood group antibodies to determine the difference of different methods. Methods: The anti-D with multiples of dilutions were detected by the method of agglutination with amines, IAT, LISS-gel and LISS-IAT respectively. IgG anti-E, anti-Lea, anti-Jka, and 87 patient samples with blood transfusions detected in the blood mismatched samples. Results: The anti-D and anti-D, LISS-IAT assay showed the highest sensitivity and the lowest polybrene concentration. For weak IgG antibody, the polybrene method could not detect the IAT, but LISS-IAT Weak antibody could be detected by both the gel method and the LISS-IAT method. The screening results of 87 patients with blood transfusion showed that the number of irregular antibodies detected by LISS-gel method and LISS-IAT method all reached 10 For example, higher than the other two methods. CONCLUSIONS: Serological antibodies such as LISS-gel or LISS-IAT should be detected by more sensitive laboratory techniques in immunological serological laboratories to ensure the safety of clinical transfusion.