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目的 :探讨外周血单个核细胞 (PBMC)膜白介素 2受体 (CD2 5 )表达在肺结核病鉴别诊断中的应用价值。方法 :用生物素 链霉亲和素 (BSA)法检测肺结核、支气管肺炎患者T细胞亚群及植物血凝素 (PHA)诱导前后CD2 5表达水平。结果 :支气管肺炎患者CD3+ 、CD4 + 、CD8+ 水平分别为 (6 2 .32± 6 .34) %、(47.5 2± 7.16 ) %、(32 .12± 6 .5 5 ) % ,CD4 + /CD8+ 比值为 1.5 2± 0 .4 3,PHA诱导前后CD2 5水平分别为 (4.5 6± 1.5 2 ) %、(35 .12± 7.2 1) %。空洞型肺结核CD3+ 、CD4 + 、CD8+ 、CD4 + /CD8+ 水平分别为 (41.13± 5 .2 5 ) %、(43.38± 5 .15 ) %、(36 .2 5± 3.4 6 ) %和 1.15± 0 .2 1,非空洞型肺结核CD3+ 、CD4 + 、CD8+ 、CD4 + /CD8+ 水平分别为 (46 .2 9± 5 .6 0 ) %、(47.2 1± 4 .86 ) %、(32 .36± 4 .0 3) %、1.4 6± 0 .2 5 ,相互比较CD3+ 、CD4 + /CD8+ 差异均有显著性 (P <0 .0 1和P <0 .0 5 )。空洞型肺结核与非空洞型肺结核患者PHA诱导前后CD2 5水平分别为 (2 .13± 1.14 ) %、(2 7.2 5± 3.5 0 ) %和 (3.4 3± 1.35 ) %、(31.14± 4 .11) % ,两者相比差异均有显著性 (P <0 .0 1)。结论 :肺结核病患者体内存在明显的细胞免疫功能紊乱 ,主要表现为CD2 5表达水平降低 ,CD2 5表达
Objective: To investigate the value of the expression of interleukin-2 receptor (CD25) in peripheral blood mononuclear cells (PBMC) in the differential diagnosis of pulmonary tuberculosis. Methods: The expression of CD25 on T cell subsets and phytohemagglutinin (PHA) in patients with pulmonary tuberculosis and bronchial pneumonia was detected by streptavidin (BSA) method. Results: The levels of CD3 +, CD4 + and CD8 + in patients with bronchopneumonia were (62.32 ± 6.34)%, (47.52 ± 7.16)%, (32.12 ± 6.55)% and CD4 + / CD8 + The ratio of CD2 5 levels was 1.5 2 ± 0. 4 3 before and after PHA induction, respectively (4.5 6 ± 1.5 2)% and (35.12 ± 7.2 1)%. The levels of CD3 +, CD4 +, CD8 + and CD4 + / CD8 + in the tuberculous meningitis group were 41.13 ± 5.25%, 43.38 ± 5.15%, 36.5 ± 3.4 6% and 1.15 ± 0 .2 1 The levels of CD3 +, CD4 +, CD8 + and CD4 + / CD8 + in non-tuberculosis pulmonary tuberculosis were (46.29 ± 5.60)%, (47.21 ± 4.86)%, (32.36 ± 4 .0 3)% and 1.4 6 ± 0. 25, respectively. The differences of CD3 + and CD4 + / CD8 + between the two groups were significant (P <0.01 and P <0.05). The levels of CD25 before and after PHA treatment in patients with tuberculosis and non-tuberculosis were (2.13 ± 1.14)%, (2.72 ± 3.5)% and (3.4 ± 1.35)%, (31.14 ± 4.11 )%, The difference between the two groups was significant (P <0.01). Conclusion: There are obvious cellular immune dysfunction in patients with pulmonary tuberculosis, which is mainly manifested as the decrease of CD2 5 expression and the expression of CD2 5