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AIM: To explore effects of exogenous and endogenous catecholamines on function of lymphocytes and primary mechanisms mediating the effects. METHODS: Splenocytes of rats were exposed to norepinephrine (NE), a- or p-adrenoceptor antagonists plus NE, or α-methyl-p-tyrosine (α-MT), and then concanavalin A (Con A)-induced interleukin-2 (IL-2) production and natural killer (NK) cell cytotoxicity were determined by MTT assay and LDH assay, respectively. RESULTS: Optical density (OD) values of NE-treated groups, which reflected IL-2 production, were 0.63, 0.61, and 0.60, respectively for 1×10-10, 1×10-9, and 1×10-8 mol/L NE. They were all significantly reduced in comparison with control value of 0.68 (P<0.01). The effect of NE was blocked by either phentolamine (an α-adrenoceptor antagonist) or propanolol (a β-adrenoceptor antagonist). OD values of α-MT, an inhibitor of tyrosine hydroxylase, at doses of 1×10-10,1×10-9, and 1×10-8 mol/L respectively were 0.71, 0.71, and 0.69, which were all notably hig
AIM: To explore effects of exogenous and endogenous catecholamines on function of lymphocytes and primary mechanisms mediating the effects. METHODS: Splenocytes of rats were exposed to norepinephrine (NE), a- or p-adrenoceptor antagonists plus NE, or a-methyl-p (ty) -induced interleukin-2 (IL-2) production and natural killer (NK) cell cytotoxicity were determined by MTT assay and LDH assay, respectively. RESULTS: Optical density (OD) values of NE-treated groups, which showed IL-2 production, were 0.63, 0.61, and 0.60, respectively for 1 × 10-10, 1 × 10-9, and 1 × 10-8 mol / L NE. They were all significantly reduced in comparison with control value of 0.68 (P <0.01). The effect of NE was blocked by either phentolamine (an α-adrenoceptor antagonist) or propanolol (β-adrenoceptor antagonist). OD values of α-MT , an inhibitor of tyrosine hydroxylase, at doses of 1 × 10-10, 1 × 10-9, and 1 × 10-8 mol / L respectively, respectively, 0.71, 0.71, and 0.69, which w ere all notably hig