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随着能源危机问题日益严重,可再生能源的研究渐渐成为目前研究的热点.微藻生物能源又以众多的优点成为目前可再生能源的研究重点.我们发现,在减氮培养下的莱茵衣藻,其油脂含量增加,Limp77基因的表达量明显下降.Limp77基因编码的是一类CCCH型锌指蛋白,具有通过与DNA、RNA结合来实现转录的调控或通过调控其它基因转录的锌指蛋白来实现转录调控的功能,极可能参与到莱茵衣藻油脂代谢调控中.通过利用RNAi干涉技术构建Limp77基因的干涉载体,并通过玻璃珠法转入莱茵衣藻(Chlamydomonas reinhardtii)2A38中,研究其与油脂相关的生理生化指标的变化.实验结果表明,Limp 77基因明显抑制莱茵衣藻油脂的积累.
With the worsening of energy crisis, the research on renewable energy has gradually become the focus of current research.Although microalgae has many advantages and become the focus of renewable energy research, we found that under the nitrogen-reducing culture, , The oil content increased, Limp77 gene expression decreased significantly.Limp77 gene encodes a class of CCCH-type zinc finger protein, with the combination of DNA, RNA to achieve transcriptional regulation or through the regulation of other genes transcription of zinc finger protein The transcriptional regulation function is very likely to be involved in the lipid metabolism regulation of Chlamydomonas reinhardtii.A RNA interference technique was used to construct the interference vector of Limp77 gene and transferred into Chlamydomonas reinhardtii 2A38 by glass bead method to study its interaction with Lipid-related physiological and biochemical changes.Experimental results show that Limp 77 significantly inhibits the accumulation of oil in Chlamydomonas reinhardtii.