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目的:研究杠板归总黄酮对抗结核药物致肝损伤小鼠的保护作用,并探索作用机制。方法:将60只小鼠随机分为6组,即正常组、模型组、葡醛内酯组(200 mg/kg)及杠板归总黄酮组(600 mg/kg,300 mg/kg,150 mg/kg)。除正常组外,其余各组均灌胃异烟肼和利福平各100 mg/kg造模,同时给予杠板归总黄酮干预,每天1次。第30天,取血和肝组织,采用生化法检测血清中ALT和AST活性;酶联免疫吸附法(ELISA)检测肝组织TNF-α、IL-1β和IL-6含量;Western Blot检测肝组织Fas表达;HE染色进行肝组织病理学检查。结果:与模型组相比,杠板归总黄酮(600 mg/kg、300mg/kg)能够明显降低肝损伤小鼠血清中ALT和AST活性,明显抑制肝组织TNF-α、IL-1β、IL-6和Fas表达,并改善肝组织病变,而杠板归总黄酮组150 mg/kg对抗结核药物致肝损伤无改善作用。结论:杠板归总黄酮可保护异烟肼和利福平联用导致的肝损伤小鼠,其机制可能与抑制Fas通路和抗炎作用有关。
Objective: To study the protective effect of the total flavonoids of Pueraria baicalensis Georgi on liver injury induced by anti-tuberculosis drugs and to explore the mechanism of action. Methods: Sixty mice were randomly divided into 6 groups: normal group, model group, glucurolactone group (200 mg / kg) and total flavonoids of Leucaena sinensis (600 mg / kg, 300 mg / kg, mg / kg). Except the normal group, the other groups were given 100 mg / kg of isoniazid and rifampicin respectively, and the total flavonoids were administered once daily. On the 30th day, the blood and liver tissues were taken out and the serum ALT and AST activities were detected by biochemical methods. The contents of TNF-α, IL-1β and IL-6 in the liver tissues were detected by enzyme-linked immunosorbent assay (ELISA) Fas expression; HE staining for liver histopathology. Results: Compared with the model group, the total flavonoids (600 mg / kg, 300 mg / kg) induced by heparin could obviously reduce the activity of ALT and AST in the serum of mice with liver injury and significantly inhibited the levels of TNF-α, IL- -6 and Fas expression, and improve the liver tissue lesions, and the total flavonoids of the plate to 150 mg / kg of anti-tuberculosis drugs caused no effect of liver injury. CONCLUSION: The total flavonoids of Ligusticum chuanxiong can protect mice from liver injury induced by isoniazid and rifampicin, which may be related to the inhibition of Fas pathway and anti-inflammatory effects.