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目的:探讨司来吉兰对PD小鼠黑质的神经保护作用并对其进行评价。方法:24只雄性C57BL小鼠被随机分成三组,即司来吉兰预处理组、模型组和对照组。分别给予司来吉兰+MPTP、MPTP和生理盐水,连续应用7天。取黑质分别行流式细胞学检查,分别测定黑质细胞凋亡率、bcl-2和bax蛋白表达的平均荧光强度以及黑质酪氨酸羟化酶(TH)免疫组化和透射电镜观察。21只雄性C57BL小鼠被随机分成司来吉兰预处理组、模型组和对照组。处理方法同前。7天后应用多巴胺转运蛋白(DAT)显像剂99Tcm-TRODAT-1对每组的纹状体DAT功能保留情况进行评价。结果:模型组黑质凋亡率高于司来吉兰预处理组和对照组(P<0.01,P<0.01),司来吉兰预处理组与对照组的凋亡率无差异(P>0.05)。与对照组比较,模型组bax高表达(P<0.01)而bcl-2低表达(P<0.01):与对照组比较,司来吉兰预处理组bax低表达(P<0.01)而bcl-2高表达(P<0.01)。司来吉兰预处理组和对照组小鼠的纹状体DAT功能无差异(P>0.05)。与对照组和司来吉兰组比较,模型组小鼠纹状体DAT功能明显降低(P<0.01,P<0.01)。结论:司来吉兰预处理对小鼠黑质产生神经保护作用。司来吉兰对MPTP处理小鼠黑质的保护作用机制之一是通过上调bcl-2基因和下调bax基因而实现的:新型DAT显像剂一99Tcm-TRODAT-1可用于PD的神经
OBJECTIVE: To investigate the neuroprotective effect of selegiline on the substantia nigra of PD mice and to evaluate the neuroprotective effect. Methods: Twenty-four male C57BL mice were randomly divided into three groups: selegiline pretreatment group, model group and control group. Patients were given selegiline + MPTP, MPTP and saline respectively for 7 consecutive days. Flow cytometry was used to detect the substantia nigra. The apoptotic rate, the average fluorescence intensity of bcl-2 and bax, and tyrosine hydroxylase (TH) immunohistochemistry and transmission electron microscopy . Twenty-one male C57BL mice were randomized to selegiline pretreatment, model and control groups. The same approach as before. Seven days later, dopamine transporter (DAT) imaging agent 99Tcm-TRODAT-1 was used to evaluate striatal DAT function retention in each group. Results: The apoptotic rate of substantia nigra in model group was higher than that of selegiline pretreatment group and control group (P <0.01, P <0.01), and there was no difference in the apoptosis rate between pregmental group and control group (P> 0.05). Compared with the control group, the expression of bax in the model group was higher (P <0.01) and lower in the bcl-2 compared with the control group (P <0.01) 2 was highly expressed (P <0.01). There was no difference in the DAT function of striatum between the pretreatment group and the control group (P> 0.05). Compared with the control group and the selegiline group, the DAT function of the striatum in the model group was significantly decreased (P <0.01, P <0.01). Conclusion: Administration of selegiline has neuroprotective effects on substantia nigra in mice. One of the protective mechanisms of selegiline on MPTP-treated substantia nigra is through up-regulation of bcl-2 gene and down-regulation of bax gene: a novel DAT imaging agent-99Tcm-TRODAT-1 can be used for PD nerve