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[目的]对定量检测生活饮用水中贾第鞭毛虫和隐孢子虫的方法进行质量控制,分析其关键控制点,评估方法的可用性。[方法]测定“滤囊过滤→免疫磁分离→荧光染色法”实验各阶段的加标回收率和不同类型水样的加标总回收率,应用危害因素分析及关键控制点HACCP分析实验过程中影响定量检测的关键因素。[结果]实验各阶段“富集浓缩→免疫磁分离→染色计数”的平均加标回收率为:贾第鞭毛虫50%→80%→97%;隐孢子虫52%→88%→95%。不同类型水样平均加标回收率为:蒸馏水加标贾第鞭毛虫39%,隐孢子虫43%;水源水加标贾第鞭毛虫38%,隐孢子虫42%;管网水加标贾第鞭毛虫50%,隐孢子虫50%。方法总计贾第鞭毛虫加标回收率为42%,隐孢子虫加标回收率为45%。[结论]滤囊过滤/免疫磁分离/荧光染色法定量检测水中贾第鞭毛虫和隐孢子虫,在采取质量控制措施以避免实验各环节的危险源后,其回收率满足美国EPA1623和我国GB/T5750方法的要求,可用于各类水样的检测。
[Objective] The research aimed to quantitatively test the quality control of Giardia and Cryptosporidium in domestic drinking water, analyze its key control points and evaluate the usability of the method. [Method] The spiked recoveries and the spiked recoveries of different types of water samples from “filtration of filter capsules to immunomagnetic separation to fluorescence staining” were determined. [Method] The HACCP analysis was used to analyze the hazard factors and the critical control points The key factors affecting quantitative testing during the process. [Result] The average recoveries of “concentration enrichment → immunomagnetic separation → dyeing count” in each stage of the experiment were: 50% → 80% → 97% of Giardia; 52% → 88% of Cryptosporidium → 95%. The average recoveries of different types of water samples were as follows: 39% of distilled water spiked with Giardia lamblia and 43% of Cryptosporidium, 38% of water-spiked with Giardia and 42% of Cryptosporidium, 50% of aliquot, 50% of Cryptosporidium. Methods The total recovery of giardiasis was 42% and the recovery of cryptosporidiosis was 45%. [Conclusion] The quantitative detection of Giardia and Cryptosporidium parvum in the water by filter media filtration / immunomagnetic separation / fluorescence staining could meet the requirements of US EPA1623 and GB / T5750 method requirements, can be used for various types of water testing.