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目的:利用正交实验筛选出三七皂苷单体最佳组合并观察其对氧化低密度脂蛋白(Ox-LDL)损伤血管内皮细胞的保护作用。方法:体外培养人脐静脉内皮细胞(HUVEC),加入100mg.L-1OX-LDL诱导单核-内皮细胞黏附,蛋白染料法测定黏附值,通过正交试验设计筛选出三七皂苷单体最佳组合;比色法检测乳酸脱氢酶(LDH)的释放量;硝酸还原法和放射免疫法分别测定HU-VEC培养上清液中一氧化氮(NO)、内皮素(ET)含量。结果:获得最佳PNS单体组合为:Rg1:Rb1:R1为1:10:1(Rg110-5mol/L、Rb110-4mol/L、R110-5mol/L),最佳单体组合显著降低LDH及ET水平,提高NO含量(P<0.01)。结论:由正交实验设计筛选出的最佳单体组合对OX-LDL所致的血管内皮细胞损伤有显著的保护作用。
OBJECTIVE: To screen out the best combination of notoginsenoside monomer by orthogonal experiment and observe its protective effect on oxidized low density lipoprotein (Ox-LDL) -induced vascular endothelial cells. Methods: Human umbilical vein endothelial cells (HUVECs) were cultured in vitro. The adhesion of mononuclear endothelial cells (HUVECs) was induced by addition of 100mg.L-1OX-LDL. The adhesion value was determined by protein dye method. The content of lactate dehydrogenase (LDH) was measured by colorimetric method. The contents of nitric oxide (NO) and endothelin (ET) in the culture supernatant of HU-VEC were determined by nitrate reduction and radioimmunoassay. Results: The best combination of PNS monomer was Rg1: Rb1: R1 was 1:10: 1 (Rg110-5mol / L, Rb110-4mol / L, R110-5mol / L) And ET levels increased NO content (P <0.01). Conclusion: The best monomer combination screened by orthogonal experimental design has a significant protective effect on endothelial cell injury induced by OX-LDL.