利用脂肪酸鉴定植物致病性细菌

• 作 者: 安德荣 魏宁生 慕小倩
• 机 构: 西北农业大学植保系,西北农业大学植保系,西北农业大学植保系,
• 出 处: 陕西农业科学
• 发表日期: 1991年01期
• 关键词: 致病性细菌 脂肪酸 致病细菌 气相色谱仪 细菌 植物组织 同源性 毛细管柱 DNA 假单胞菌

利用整个细胞的脂肪酸对细菌进行分类已有20多年了,而用其对植物致病性细菌的鉴定只是近两年出现的新技术。利用脂肪酸所鉴定的结果和用互补核酸探针测定细菌之间的DNA同源性的结果一致。方法是从细菌纯培养的湿细胞或受侵植物组织中直接提取植物致病细菌的脂肪酸,利用任何型号的气相色谱仪进行分析。由于毛细管柱(50％苯甲基硅)有很好的色谱峰,所以在没有计算机的情况下,可以

Bacteria have been used for more than 20 years to classify fatty acids in whole cells, and their identification of phytopathogenic bacteria is only new technology that has emerged in the past two years. The results identified with fatty acids are consistent with the results of the DNA homology determination between bacteria using complementary nucleic acid probes. The method is to directly extract the fatty acids of plant pathogenic bacteria from bacterial purely cultured wet cells or tissue of infested plants and analyze with any type of gas chromatograph. Due to the good chromatographic peaks of the capillary column (50% phenylmethylsilicone), in the absence of a computer,