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利用酶抑制技术检测微量有机磷农药,一般均采用牛、鼠、羊、兔、猴、鸡、猪等动物酶源,鉴于植物种子、根、茎、叶、花、果实中含有的水解酶,同动物酯酶一样,其活力亦能被有机磷所抑制,作者摸索了面粉等植物酶在薄层板上进行酶化学反应来检测甲胺磷残留量的可行性,结果令人满意。 1.方法原理:甲胺磷在薄层板上被展开后,喷洒植物酶溶液,在斑点部位植物酶被农药抑制,而在斑点之外,酶的活性未被抑制,生成了基质水解产物,与特定试剂呈现有色反应,因此,在喷洒基质溶液后,斑点是白色,而背景显玫瑰红色。
Detection of trace organophosphorus pesticides by enzyme inhibition technology generally uses animal enzyme sources such as cattle, rats, sheep, rabbits, monkeys, chickens and pigs. In view of the hydrolase contained in plant seeds, roots, stems, leaves, flowers and fruits, Like the animal esterase, its activity can also be inhibited by organophosphorus. The author explored the feasibility of detecting the residues of methamidophos by enzymatic chemical reactions on the laminae such as flour and other plant enzymes, with satisfactory results. 1. Method principle: methamidophos was spread on a thin plate, spraying the plant enzyme solution, the enzyme in the spot site was inhibited by pesticides, and in the spots, the enzyme activity was not inhibited, resulting in matrix hydrolyzate, Colored reaction with a specific reagent, therefore, after spraying the matrix solution, the spots are white and the background is rosy.