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目的 巨细胞病毒广泛存在 ,人和动物感染后可导致多种血管疾病。血栓闭塞性脉管炎 ,作为一种血管疾病 ,其病因未明 ,本研究的目的在于考察该病与巨细胞病毒感染的相关性。 方法 为了考查血栓闭塞性脉管炎病变血管壁中巨细胞病毒DNA的存在 ,以PCR方法分析了 41例该病血管壁标本和 14例正常血管壁标本 ,引物基于该病毒即刻早期基因上游增强子的核酸序列。 结果 41例病变血管标本中有 2 8例 (6 9.2 9% )发现巨细胞病毒核酸的存在 ,14例正常血管壁中有 2例 (14.2 9% )。两组间巨细胞病毒的感染率有统计学差异 (P <0 .0 5 )。血栓闭塞性脉管炎病变血管壁的部位与巨细胞病毒感染率无关 (P >0 .0 5 )。血栓闭塞性脉管炎吸烟患者与非吸烟患者病变血管壁中巨细胞病毒感染率无统计学差异 (P >0 .0 5 )。 结论 结果显示 ,在血栓闭塞性脉管炎病变血管壁中存在巨细胞病毒感染 ,它的感染可能与该病的发生和 (或 )发展有关
The purpose of CMV widespread, human and animal infections can lead to a variety of vascular disease. Thromboangiitis obliterans, as a vascular disease, the etiology is unknown, the purpose of this study is to examine the relationship between the disease and cytomegalovirus infection. Methods In order to investigate the presence of cytomegalovirus DNA in the vascular wall of thromboangiitis obliterans, 41 specimens of vascular wall and 14 normal vessels were analyzed by PCR. The primers were based on the immediate upstream gene enhancer Of the nucleic acid sequence. RESULTS: Twenty-eight cases (69.29%) of the 41 lesions showed the presence of cytomegalovirus nucleic acid. There were 2 cases (14.2%) of 14 normal vessel walls. The infection rate of cytomegalovirus between the two groups was statistically significant (P <0.05). Thromboangiitis obliterans had no correlation with cytomegalovirus infection (P> 0.05). There was no significant difference in the infection rate of cytomegalovirus in the vessel wall of thromboangiitis obliterans and non-smoking patients (P> 0.05). Conclusions The results showed that there is cytomegalovirus infection in the vascular wall of thromboangiitis obliterans and its infection may be related to the occurrence and / or development of the disease