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细胞中普遍存在各种颗粒结构,在不同的生理、病理与实验条件下,颗粒的形状、大小与数目,都可能发生变化。在立体学中,对于形状不规则的颗粒,可以定量地描述其形状,但一般难以得到其大小与数目的参数。最近发展起来一种新方法,可以在细胞超薄切片的电镜图像上直接测算不规则颗粒的体积。我们使用正方测试格来测量凸形与非凸形颗粒的体积,得去了具体的测算步骤。对于其中的规则颗粒和我们推导出来的截距法所得到的数据相比,结果十分吻合。对于凸形颗粒,以落在颗粒截面内的测试点为采样点,遍过每个点有纵横各一条截距。若在某种颗粒截面内共有k个测试点,则共需测量2k条截距。若某条截距内有n个测试点,则这条截距应取n次。颗粒
Cells generally exist in a variety of particle structure, in different physiological, pathological and experimental conditions, the shape of the particles, size and number may change. In stereology, the shapes of irregularly shaped particles can be quantitatively described, but it is generally difficult to obtain the parameters of their size and number. A new method has recently been developed that directly measures the size of irregular particles on electron micrographs of ultrathin sections of cells. We use square test cells to measure the volume of convex and non-convex particles, to go to a specific measurement step. The results are in good agreement with the data obtained from the intercepts of the regular particles and the one we derived. For convex particles, fall into the particle cross-section of the test point for the sampling points, each point has a vertical and horizontal intercept. If there are k test points in a certain particle cross-section, a total of 2k intercepts must be measured. If there is n test points within an intercept, this intercept should be taken n times. Granules