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目的观察从东亚钳蝎中提取的神经元营养活性肽(NNAP)对帕金森病(PD)大鼠的保护作用及其对小胶质细胞的影响。方法采用6-羟多巴胺(6-OHDA)制备PD大鼠模型,通过行为学检测和中脑黑质致密部的酪氨酸羟化酶(TH)以及OX-42的免疫组织化学染色,同时设立实验给药组,观察NNAP对PD大鼠的旋转行为以及黑质DA能神经元和小胶质细胞的影响。结果 经ip NNAP[蛋白浓度为0.05 mg/(kg.d)]处理7 d后的PD大鼠旋转次数较模型组明显减少(P<0.01)。在6-OHDA大鼠损毁侧,实验给药组与模型组相比,在黑质处20 000μm2测量面积内,DA能神经元的免疫反应阳性神经元数目和吸光度与模型组比较明显增高(P<0.01);小胶质细胞的数目和吸光度与模型组比较明显降低(P<0.01)。结论 NNAP对PD大鼠具有保护作用,抑制小胶质细胞免疫反应活性可能参与其机制。
Objective To observe the protective effect of neuronal nutrition peptide (NNAP) extracted from Scorpio in East Asia on Parkinson’s disease (PD) rats and its effect on microglia. Methods PD rat models were prepared by 6-hydroxydopamine (6-OHDA), and immunohistochemical staining of OX-42 and tyrosine hydroxylase (TH) in substantia nigra pars compacta The experimental group was treated with NNAP to observe the effects of NNAP on the rotational behavior of PD rats and the DA neurons and microglia in substantia nigra. Results The number of rotations of PD rats treated with ip NNAP [protein concentration of 0.05 mg / (kg · d)] for 7 days was significantly lower than that of the model group (P <0.01). Compared with the model group, the number of immunoreactive neurons and the absorbance of DA neurons in the experimental group were significantly higher than those in the untreated group (P <0.01). The numbers and absorbance of microglia were significantly lower than those in model group (P <0.01). Conclusion NNAP has a protective effect on PD rats and inhibits microglial immunoreactivity may be involved in the mechanism.