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产肠毒索大肠杆菌(ETEC)是急性腹泻的主要病原菌,已引起全世界重视。我们自1974年首次发现ETEC后,经过10多年调查研究,基本摸清我省ETEC的流行概况,现报告如下。 材料与方法 一、调查对象和方法 在我省各级医疗单位就诊的腹泻患者和当地健康人群,采集粪便保存于Cary—Blair培基中,当日送实验室分离培养。粪便标本直接接种美兰琼脂平板,挑取疑为大肠杆菌者3~4株,经生化鉴定,证实为大肠杆菌后,
Enterotoxigenic Escherichia coli (ETEC) is the main pathogen of acute diarrhea, has attracted worldwide attention. After we first discovered ETEC in 1974, after more than 10 years of investigation and study, we have basically found out the epidemic situation of ETEC in our province. The report is as follows. MATERIALS AND METHODS A. Subjects and Methods The diarrhea patients and local healthy people from medical units at all levels of the province were collected for collecting feces in Cary-Blair culture medium and sending them to laboratory for separation and culture on the same day. Stool samples were inoculated with Meilan agar plate, pick the suspected Escherichia coli 3 to 4 strains, biochemical identification, confirmed as E. coli,