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为探讨复方黄连降糖片(主药:大黄、黄连、肉桂等)对胰岛素抵抗型KK ay小鼠骨骼肌葡萄糖转运蛋白4(glucosetransporter 4,GLUT 4)基因表达的影响,将雄性KK ay小鼠,随机分为模型组、中药低剂量组、中药高剂量组、西药组,饲以高脂饮食;选用健康的雄性C57BL小鼠作对照,饲以普通饮食。用药两周后处死动物,测定各组的空腹血糖、血脂、血清胰岛素、GLUT 4mRNA表达。结果:KK ay小鼠在饲以高脂饮食后,血糖、血脂、血清胰岛素显著升高,同时骨骼肌GLUT 4mRNA的表达明显下降。治疗后血糖降低,血甘油三酯降低,骨骼肌GLUT 4mRNA表达明显增加。结论:复方黄连降糖片能改善胰岛素抵抗型KK ay小鼠的糖脂代谢紊乱,增加KK ay小鼠的骨骼肌GLUT 4mRNA表达。
To investigate the effects of compound Huanglian Jiangtang tablets (main drugs: rhubarb, berberine, cinnamon, etc.) on gene expression of skeletal muscle glucose transporter 4 (GLUT4) in insulin resistant KK ay mice, male KK ay mice were studied. They were randomly divided into model group, low-dose Chinese medicine group, high-dose Chinese medicine group, and western medicine group, fed with a high-fat diet; healthy male C57BL mice were used as controls and fed with normal diet. Two weeks later, animals were sacrificed and fasting blood glucose, blood lipids, serum insulin, and GLUT4 mRNA expression were measured in each group. RESULTS: After KK ay mice were fed a high-fat diet, blood glucose, blood lipids, and serum insulin were significantly increased, and the expression of GLUT4 mRNA in skeletal muscle was significantly decreased. After treatment, blood glucose decreased, blood triglyceride decreased, and the expression of GLUT4 mRNA in skeletal muscle increased significantly. Conclusion: Compound Huanglian Jiangtang Tablet can improve the disorder of glucose and lipid metabolism in insulin resistant KK ay mice and increase the expression of GLUT 4 mRNA in KK ay mice.