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本研究以木薯品种新选048诱导膨大的茎尖分生组织为外植体,研究了不同浓度植物生长调节剂、不同碳源、不同成熟时间的体细胞胚及其子叶切片对木薯体细胞胚发生及植株再生的影响。结果表明,与2,4-D相比,12 mg/L的Picloram诱导木薯体细胞胚发生率最高,为36.2%,其产胚量为5.87;不同碳源对木薯体细胞胚继代增殖无显著性差异;随着体细胞胚成熟时间的增加,其植株的再生率也随之升高,但后期差异不显著;不同成熟时间的体细胞胚子叶器官发生率差异显著,诱导成熟14 d左右的子叶切片植株再生率较高,诱导率达54.3%。
In this study, cassava variety newly selected 048 induced expansion of shoot apical meristem as explants to study the different concentrations of plant growth regulators, different carbon sources, different maturation time of somatic embryos and cotyledons section of cassava somatic embryos Occurrence and plant regeneration. The results showed that compared with 2,4-D, picloram at 12 mg / L induced the highest somatic embryogenesis rate of cassava (36.2%) and its embryogenic yield was 5.87. The effects of different carbon sources on somatic embryogenesis of cassava Significant difference; With the maturation of somatic embryos maturation time, the plant regeneration rate also increased, but the late difference was not significant; different maturation time somatic embryo cotyledons organs significantly different, induction of maturity about 14 d Of the cotyledons plant regeneration rate higher, the induction rate of 54.3%.