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WRKY蛋白是只存在于植物中的一类转录因子家族,由WRKY蛋白N端高度保守的WRKYGQK氨基酸序列及特殊的锌指结构而命名。WRKY除参与植物发育和代谢的调控外还与植物的抗逆反应有关。本研究利用抗病相关基因NPR1基因启动子区的W-box顺式元件采用酵母单杂交方法,以拟南芥At NPR1启动子区域W-box元件构建诱饵载体,从大豆中分离到了一个转录因子Gm WRKY53,通过序列分析表明,Gm WRKY53具有与At WRKY蛋白的保守氨基酸序列极相似的二级结构,在酵母单杂交系统中该蛋白能够与抗病相关基因At NPR1基因启动子区的W-box特异结合并启动报道基因的表达。对大豆WRKY转录因子的研究有助于深入理解大豆抗病及发育调控机制。
The WRKY protein is a family of transcription factors that exist only in plants and is named by the WRKYGQK amino acid sequence that is highly conserved at the N-terminus of WRKY proteins and a special zinc finger structure. In addition to WRKY involved in the regulation of plant development and metabolism but also with the plant’s anti-retrogradation reaction. In this study, the W-box cis-element in the promoter region of the disease-related gene NPR1 gene was used to construct the bait vector by using the yeast single hybridization method and the bait vector was constructed from the W-box element of the Arabidopsis AtNPR1 promoter region. A transcription factor Gm WRKY53. The sequence analysis showed that Gm WRKY53 has a secondary structure very similar to the conserved amino acid sequence of At WRKY protein, which can interact with the W-box of the promoter region of At NPR1 gene in the yeast single hybrid system Specifically binds and initiates the expression of the reporter gene. The study of WRKY transcription factor in soybean helps to understand the mechanism of soybean disease resistance and development.