【摘 要】
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Purpose:To investigate the inhibitory effect of diclofenac sodium on rabbit corneal epithelial cells(RCECs)in vitro and explore its pharmacological mechanism.Me
【机 构】
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Eye Hospital of China Academy of Chinese Medical Sciences,Ophthalmic Center
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Purpose:To investigate the inhibitory effect of diclofenac sodium on rabbit corneal epithelial cells(RCECs)in vitro and explore its pharmacological mechanism.Methods: The fresh rabbit cornea was cultured to get the primary RCECs,and RCECs of passage 2 were used for the research.The cells were divided into experimental groups,the cells in which were incubated with different concentrations(18.18,27.27,36.36,45.45,54.55 μg/ml)of dielofenac sodium,and control group.The effect of diclofenac sodium on the proliferation of cells was measured by methyl thiazolyl thiazolium(MTT)assay 24,48 and 72 h after incubation.While the RCECs were divided into experimental groups,the cells in which were incubated with 9 and 12.5μg/ml diclofenac sodium,and control group.The cell cycle and apoptotic rate were observed by flow cytometer.Results:MIT assay showed that diclofenac sodium had obvious inhibitory effect on RCECs,and the inhibition rate was increasing along with the increase of the concentration of diclofenac sodium and the incubation time(P
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