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采用微波辅助顶空固相微萃取法(MAE-HS-SPME)分析葎草不同部位的挥发油化学成分,通过气相色谱-质谱法与Kovats色谱保留指数相结合进行定性定量分析,以峰面积归一化法计算各组分的相对质量分数.结果表明,在葎草的茎、叶和花中分别鉴定出86、90和103个组分,分别占挥发油总峰面积的96.697%、96.455%和94.213%;在茎中主要成分是1,3-二甲基双环[3.3.0]辛-3-烯-2-酮(14.010%),百里酚(9.407%),β-石竹烯(6.884%),反式-β-金合欢烯(5.965%),β-甜没药烯(5.798%),香芹酚(5.702%)和α-佛手柑油烯(5.511%);叶中主要成分是β-石竹烯(12.704%),α-佛手柑油烯(8.588%),1,3-二甲基双环[3.3.0]辛-3-烯-2-酮(7.487%)和反式-β-金合欢烯(6.662%);在花中主要是反式-β-金合欢烯(11.906%),β-石竹烯(8.201%),α-葎草烯(5.515%)和β-蒎烯(4.719%);而相对质量分数有显著差异.
The chemical constituents of volatile oil from different parts of Artemisia sphaerocephala were analyzed by microwave-assisted headspace solid-phase microextraction (MAE-HS-SPME). The chemical constituents were identified by gas chromatography-mass spectrometry combined with Kovats retention index. And the relative mass fraction of each component was calculated.The results showed that 86, 90 and 103 components were identified in stem, leaf and flower respectively, accounting for 96.697%, 96.455% and 94.213, respectively, of the total peak area of volatile oil %; The main components in the stem are 1,3-dimethylbicyclo [3.3.0] oct-3-en-2-one (14.010%), thymol (9.407% ), Trans-β-farnesene (5.965%), β-melatonin (5.798%), carvacrol (5.702%) and α-bergamotenoid (5.511%); α-bergapten (8.588%), 1,3-dimethylbicyclo [3.3.0] oct-3-en-2-one (7.487%) and trans- (6.662%) of β-farnesene; mainly trans-β-farnesene (11.906%), β-caryophyllene (8.201%), α-humulene (5.515% Alkene (4.719%), while the relative mass fraction was significantly different.