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[Objective] To analyze the podophyllotoxin in different parts of Dysosma tsayuensis Ying by HPLC,and the contents of which were determined.[Method] The chromatographic conditions were as follows:chromatographic column of Whates C18 column(125.0 mm×4.6 mm,5 μm),mobile phase of methanol and water in 55:45 volume ratio,flow rate of 1.0 ml/min,detection wavelength of 290 nm,column temperature of 25 ℃ and sampling volume of 20 μl.The regression equation was obtained with the concentration of reference solution x ranged from 0 to 200 μg/ml:y=15 121x-46 585,r=0.999 1.[Result] The podophyllotoxin could be detected in the root,tuber,leaf,petiole,flower and fruit of Dysosma tsayuensis Ying,while its contents varied significantly in different parts.The content of podophyllotoxin decreased in the following order,tuber,root,flower,leaf,petiole and fruit,which were 180.50±2.74,58.83±1.59,40.33±1.24,36.93±1.09,29.90±0.45 and 6.03±0.12(md) μg/g,respectively.[Conclusion] The method was characterized by high sensitivity,good stability,high repeatability and good separating effect.Podophyllotoxin content was the highest in tuber and the lowest in fruit of Dysosma tsayuensis Ying.This research would provide a theoretical and practical direction for analyzing efficiency constituents in medicinal plants of Tibet.
[Objective] To analyze the podophyllotoxin in different parts of Dysosma tsayuensis Ying by HPLC, and the contents of which were determined. [Method] The chromatographic conditions were as follows: chromatographic column of Whates C18 column (125.0 mm × 4.6 mm, 5 μm ), mobile phase of methanol and water in 55:45 volume ratio, flow rate of 1.0 ml / min, detection wavelength of 290 nm, column temperature of 25 ° C and sampling volume of 20 μl. The regression equation was obtained with the concentration of Reference solution x ranged from 0 to 200 μg / ml: y = 15 121x-46 585, r = 0.999 1. [Result] The podophyllotoxin could be detected in the root, tuber, leaf, petiole, flower and fruit of Dysosma tsayuensis Ying , while its contents varied significantly in different parts. The content of podophyllotoxin decreased in the following order, tuber, root, flower, leaf, petiole and fruit, which were 180.50 ± 2.74, 58.83 ± 1.59, 40.33 ± 1.24, 36.93 ± 1.09, 29.90 ± 0.45 and 6.03 ± 0.12 (md) μg / g, respectively. [Conclusion] The method was cha racterized by high sensitivity, good stability, high repeatability and good separating effect. Podophyllotoxin content was the highest in tuber and the lowest in fruit of Dysosma tsayuensis Ying. This research would provide a theoretical and practical direction for analyzing efficiency constituents in medicinal plants of Tibet .