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目的介绍验证转运体稳定表达的方法和技术,为转运体细胞模型的应用提供依据。方法对近年来研究转运体在细胞模型中稳定表达的相关文献进行综述。结果实时定量PCR及Western blot是分别验证转运体转录水平和蛋白表达的经典方法。结论与荧光检测技术相结合的验证方法在转录水平、蛋白表达与功能活性等三方面都显示出广阔的应用前景。
Objective To introduce the methods and techniques for verifying the stable expression of transporters and provide the basis for the application of transporter somatic cell models. Methods The related literatures about the stable expression of transporters in cellular models in recent years were reviewed. Results Real-time quantitative PCR and Western blot were the classical methods to verify the transcriptional level and protein expression of transporter respectively. Conclusion The verification method combined with fluorescence detection technology shows a broad application prospect in three aspects: transcription level, protein expression and functional activity.