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目的通过生物信息学分析预测牛乳腺生物反应器表达的重组人α-乳清白蛋白(rhLA)的致敏性。方法使用在线致敏原数据库(The Allergen Online Database)、致敏蛋白结构数据库(Structural Database of Allergenic Proteins)和食品安全致敏原数据库(Allergen Database for Food Safety),分析目标蛋白与已知致敏原的序列或结构相似性。结果 rhLA致敏性的生物信息学分析结果显示,rhLA与已知致敏原牛α-乳清白蛋白、牛乳糖合酶B蛋白(致敏原Bos d 4)、鸡溶菌酶C(1,4-β-N乙酰胞壁质酶C、致敏原Gal d IV、致敏原Gal d 4)存在较高的序列或结构同源性。结论 rhLA具有潜在的致敏可能性。
OBJECTIVE: To predict the allergenicity of recombinant human α-lactalbumin (rhLA) expressed in bovine mammary gland by bioinformatics analysis. Methods The Allergen Online Database, the Structural Database of Allergenic Proteins and the Allergen Database for Food Safety were used to analyze the relationship between the target protein and known allergens Sequence or structural similarity. Results Bioinformatics analysis of sensitization to rhLA showed that rhLA was highly sensitive to the known allergens of the cow α-lactalbumin, bovine lactate synthase B protein (allergen Bos d 4), chicken lysozyme C (1,4 -β-N acetyltransferase C, the allergen Gal d IV, the allergen Gal d 4) have high sequence or structural homology. Conclusion rhLA has the potential of sensitization.