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研究ｒａｓｐ２１和ｐ５３表达和ＤＮＡ倍体异常与尿路上皮肿瘤发生关系。方法：应用流式细胞术和细胞免疫荧光技术对５２例尿路上皮肿瘤ｒａｓｐ２１和ｐ５３蛋白和ＤＮＡ含量进行定量检测。结果：异倍体２２例（４２．３％），二倍体３０例（５７．７％）；异倍体肿瘤其ＤＮＡ指数、增殖指数和Ｓ期细胞比率均高于二倍体肿瘤，但差异无显著性（Ｐ＞０．０５）；ｒａｓｐ２１和ｐ５３共同表达２５例（４８．１％），随肿瘤病理分级分期的上升而增加（Ｐ＜０．０５）；异倍体肿瘤ｒａｓｐ２１和ｐ５３共同表达１６例（７２．７％），二倍体肿瘤ｒａｓｐ２１和ｐ５３共同阳性９例（３０％），两组间差异显著（Ｐ＜０．０１）。结论：ＤＮＡ倍体异常、ｒａｓｐ２１和ｐ５３的共同表达参与尿路上皮肿瘤的发生和发展。||关键词##4尿路上皮肿瘤;;ｒａｓｐ２１;;ｐ５３;;ＤＮＡ倍体;;流式细胞术
To investigate the relationship between rasp21 and p53 expression and DNA ploidy abnormalities and urothelial tumors. Methods: Flow cytometry and immunofluorescence were used to detect the protein and DNA content of rasp21 and p53 in 52 cases of urothelial tumors. Results: aneuploidy was found in 22 cases (42.3%) and diploid in 30 cases (57.7%). The DNA index, proliferation index and S phase of aneuploid tumors were higher than that of diploid tumors There was no significant difference between the two groups (P> 0.05). The expression of rasp21 and p53 in 25 cases (48.1%) was increased with the increase of tumor stage (P <0.05) 16 cases (72.7%) were co-expressed, 9 cases (30%) were positive for diploid tumor rasp21 and p53, and there was significant difference between the two groups (P <0.01). Conclusion: DNA ploidy abnormality, rasp21 and p53 co-expression involved in the occurrence and development of urothelial tumors. || Keywords ## 4 urothelial tumors ;; rasp21 ;; p53 ;; DNA ploidy ;; flow cytometry