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AIM:To investigate the effect of Tripterygiumhyp-oglaucum Hutch(THH)on the assembly anddisassembly process of tubulin and its possible mode ofaction.METHODS:In vitro porcine brain tubulin assemblyassay was employed to analyze the inhibitory effects ofTHH at different concentrations(0.05 μg/L,0.07 μg/L,0.09μg/L).Colchicine(0.0025 mmol/L,0.0050 mmol/L,0.0075mmol/L)was used as a positive control.RESULTS:THH could significantly inhibit the assembly ofisolated porcine brain tubulin at all tested concentrations.CONCLUSION:THH is capable of inducing aneuploidyin mammals via tubulin polymerization inhibition pathwayand may pose a genetic risk to human beings.
AIM: To investigate the effect of Tripterygiumhyp-oglaucum Hutch(THH) on the assembly anddisassembly process of tubulin and its possible mode of action.METHODS:In vitro porcine brain tubulin assemblyassay was employed to analyze the inhibitory effects ofTHH at different concentrations(0.05 μg/ L, 0.07 μg/L, 0.09 μg/L). Colchicine (0.0025 mmol/L, 0.0050 mmol/L, 0.0075 mmol/L) was used as a positive control. RESULTS: THH could significantly inhibit the assembly of isolated porcine brain tubulin at All tested concentrations.CONCLUSION:THH is capable of inducing aneuploidyin mammals via tubulin polymerization inhibition pathway and may pose a genetic risk to human beings.