论文部分内容阅读
目的:探讨炎症及出血导致流产的作用机制。方法:选择复发性流产(RSA)及正常妊娠孕6~10周的蜕膜组织各10例,并在体外分离培养正常妊娠的蜕膜细胞,加入雌、孕激素、白介素β1(IL-β1)及凝血酶处理后,用实时荧光定量RT-PCR及Western blotting检测RSA患者、正常妊娠者蜕膜组织及培养处理的各组蜕膜细胞内的同源框A10(HOXA10)的表达情况。结果:1HOX A10基因在孕早期蜕膜细胞中有表达;与正常妊娠者相比,RAS患者蜕膜组织HOXA10的表达明显下降(P<0.05)。2雌、孕激素处理组的蜕膜细胞HOXA10 mRNA的表达显著增加(P<0.05),是未处理组的9.5倍。3进一步加入IL-β1或凝血酶后,HOXA10 mRNA表达显著下降(P<0.05);与雌、孕激素组相比,分别下降89.5%和74.9%。结论:1 HOXA10基因在孕早期蜕膜细胞中有显著表达,在RAS患者中的表达显著下降。2雌、孕激素促进HOXA10的表达,而IL-β1及凝血酶抑制HOXA10的表达。
Objective: To explore the mechanism of inflammation and bleeding induced abortion. Methods: Ten cases of recurrent spontaneous abortion (RSA) and normal decidual tissue from 6 to 10 weeks of gestational pregnancy were selected and normal pregnant decidual cells were isolated and cultured. Estrogen and progesterone, interleukin -β1 (IL-β1) And thrombin treatment, the expression of homeobox A10 (HOXA10) in the decidual cells of RSA patients, normal pregnancy decidual tissues and cultured decidual cells was detected by real-time fluorescent quantitative RT-PCR and Western blotting. Results: 1HOX A10 gene was expressed in early pregnancy decidual cells. Compared with normal pregnancy, HOXA10 expression in decidual tissue of RAS patients was significantly decreased (P <0.05). The expression of HOXA10 mRNA in decidual cells of estrogen and progesterone treatment groups was significantly increased (P <0.05), which was 9.5 times higher than that of untreated group. 3 After the addition of IL-β1 or thrombin, the expression of HOXA10 mRNA decreased significantly (P <0.05), and decreased by 89.5% and 74.9% respectively compared with the control group. Conclusion: 1 HOXA10 gene in early pregnancy decidual cells were significantly expressed in RAS patients decreased significantly. 2 estrogen and progesterone promote HOXA10 expression, while IL-β1 and thrombin inhibit HOXA10 expression.