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目的探讨米诺环素对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的C57BL/6小鼠的行为学损伤的保护作用及其机制。方法通过MPTP腹腔注射C57BL/6小鼠建立帕金森病亚急性模型。40只C57BL/6雄性小鼠随机分为对照(control)组,模型(MPTP)组,治疗(MPTP+Mino)组和米诺环素阴性对照(Mino)组,每组10只。分别采用游泳、爬杆和自主活动计数实验检测小鼠的运动行为能力。并采用HPLC测定中脑多巴胺(DA)、二羟苯乙酸(DOPAC)和高香草酸(HVA)的含量。结果治疗组游泳评分,爬杆评分和自主活动能力明显高于模型组(P<0.01)。且治疗组的中脑DA、DOPAC和HVA的含量明显高于模型组(P<0.01)。结论米诺环素对MPTP所致的小鼠运动功能损伤具有良好的保护作用,其机制可能是通过抑制MPTP所致的小鼠多巴胺神经元的损伤而起作用。
Objective To investigate the protective effect and mechanism of minocycline on behavioral injury induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in C57BL / 6 mice. Methods Subacute Parkinson’s disease was induced by intraperitoneal injection of MPTP into C57BL / 6 mice. Forty C57BL / 6 male mice were randomly divided into control group, MPTP group, MPTP + Mino group and Mino group, 10 mice in each group. Swimming, climbing pole and independent activity counting test were used to detect the motor performance of mice. The levels of midbrain dopamine (DA), dihydroxybenzene acetic acid (DOPAC) and homovanillic acid (HVA) were determined by HPLC. Results The scores of swimming, climbing score and voluntary activities in the treatment group were significantly higher than those in the model group (P <0.01). The contents of DA, DOPAC and HVA in the midbrain of the treatment group were significantly higher than those in the model group (P <0.01). Conclusion Minocycline has a good protective effect on motor function impairment induced by MPTP in mice, and its mechanism may be through inhibiting MPTP-induced damage of dopamine neurons in mice.