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目的:探讨黄芪配伍不同剂量柴胡对脾虚发热大鼠模型的作用。方法:雄性Wistar大鼠随机分为正常对照组、脾虚发热模型组、芪柴高、中、低剂量组、高剂量柴胡组共6组。采用饮食失节+游泳疲劳制备脾虚模型。实验第17天开始,各给药组大鼠分别按剂量4.56,2.95,2.41,2.41 g·kg-1ig,正常组和模型组同体积蒸馏水ig,1次/d,连续6 d。第22天模型组和各给药组以脂多糖(LPS,80μg·kg-1)ip,正常对照组等量生理盐水ip。观测各组大鼠每周的外观行为变化和LPS ip后不同时间点的体温,测定外周血相关脑肠肽的含量。结果:与正常组相比,模型组大鼠在LPS ip后各点体温均见不同程度升高,60,220 min时升高明显(P<0.05);外观行为积分显著升高(P<0.05),外周血生长抑素(SS)、胆囊收缩素(CCK-8)、β内啡肽(β-EP)均显著升高(P<0.05)。与脾虚发热组相比,各给药组大鼠体温均见不同程度下降(P<0.05),其中柴胡组和芪柴中、低剂量组体温下降明显,体温曲线明显偏离正常组;各给药组中仅芪柴中、低剂量组的部分行为指标及积分下降明显(P<0.05);各给药组血β-EP均显著升高(P<0.05),芪柴各剂量组血SS和CCK-8均呈不同程度的降低作用趋势,但高剂量柴胡组SS和CCK-8反见升高趋势。与高剂量柴胡组比较,芪柴各剂量组血SS均显著降低(P<0.05),芪柴高剂量组血β-EP显著降低(P<0.05),芪柴中、低剂量组血CCK-8显著降低(P<0.05)。结论:黄芪配伍不同剂量柴胡对脾虚发热模型均有退热作用,对外观行为或相关脑肠肽均有不同程度的改善作用;单用高剂量柴胡仅有退热作用;综合效应以黄芪配伍低剂量柴胡的防治效果最好。
Objective: To investigate the effect of Radix Astragali combined with different doses of Bupleurum on rat model of spleen-deficiency fever. Methods: Male Wistar rats were randomly divided into normal control group, spleen deficiency fever model group, Qichai high, medium and low dose groups and high dose Bupleurum group. The model of spleen deficiency was made by dietary nocturnal + swimming fatigue. At the 17th day of experiment, the rats in each administration group were treated with 4.56, 2.95, 2.41 and 2.41 g · kg-1 doses respectively, and the normal and model groups were distilled water ig once a day for 6 days. On the 22nd day, the rats in the model group and each administration group were given ip (80μg · kg-1) lipopolysaccharide (LPS) and normal saline (ip). The appearance and behavior of rats in each group were observed and the body temperature at different time points after LPS ip was measured to determine the content of gut peptide in peripheral blood. Results: Compared with the normal group, the body temperature of rats in model group increased to different degrees after LPS ip, but increased significantly at 60,220 min (P <0.05), and the scores of appearance behavior increased significantly (P <0.05) The levels of somatostatin (SS), cholecystokinin (CCK-8) and β-EP in peripheral blood were significantly increased (P <0.05). Compared with the spleen-febrile group, the body temperature of rats in each administration group decreased to different extents (P <0.05), and the body temperature in Bupleurum chinense and Qichai medium and low dose groups decreased significantly and the body temperature curve deviated significantly from the normal group (P <0.05). The serum levels of β-EP were significantly increased in each dose group (P <0.05). The serum levels of SS And CCK-8 showed a decreasing trend to some extent, but high dose Bupleurum group SS and CCK-8 reverse trend. Compared with the high-dose Bupleurum group, the blood levels of SS in each dose group were significantly decreased (P <0.05), and the β-EP levels in the high dose QCHI group were significantly decreased (P <0.05) -8 significantly decreased (P <0.05). Conclusions: Radix Astragali combined with different doses of Bupleurum have antipyretic effect on the model of spleen deficiency fever, and have different effects on appearance behavior or related to ghrelin; single high-dose Bupleurum only has antipyretic effect; Astragalus Compatibility of low-dose Bupleurum best control effect.