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目的:研究新合成产物乙酰半胱氨酸镁盐-甘草酸环状样二聚体(acetylcysteine magnesiumglycyrrhizin fine antipode dimers,GFA-NAC·Mg)治疗大鼠肝纤维化的效果。方法:25%四氯化碳(CCl4)制备大鼠肝纤维化模型,GFA-NAC·Mg按25、50、100mg/kg剂量每日分别腹腔注射进行肝纤维化治疗,给药8周后处死大鼠,用肝纤维化网格染色观察肝纤维化情况,ELISA法测定血清羟脯氨酸(Hyp)和肝组织丙二醛(MDA)、超氧化物歧化酶(SOD)、转化生长因子-β1(TGF-β1)、血小板衍生生长因子(PDGF)。结果:大鼠肝组织病理结果显示其肝纤维化处于Ⅲ-Ⅳ期;与模型组比较,GFA-NAC·Mg治疗后血清Hyp明显降低;肝组织抗氧化指标SOD显著升高,MDA明显下降;肝纤维化指标TGF-β1、PDGF在组织含量下降;GFA-NAC·Mg各组网格染色显示肝纤维化好转。结论:GFA-NAC·Mg对肝纤维化大鼠有较好实验治疗作用,可能通过抑制PDGF和TGF-β1的表达而实现。
Objective: To study the effect of acetylcysteine magnesiumglycyrrhizin fine antipode dimers (GFA-NAC · Mg), a new synthetic product, on hepatic fibrosis in rats. Methods: Rat model of hepatic fibrosis was induced by 25% carbon tetrachloride (CCl4). GFA-NAC · Mg was injected intraperitoneally at 25, 50 and 100mg / kg respectively for hepatic fibrosis. Rats were stained with hepatic fibrosis mesh to observe the changes of hepatic fibrosis. Serum hydroxyproline (Hyp) and malondialdehyde (MDA), superoxide dismutase (SOD), transforming growth factor - β1 (TGF-β1), platelet-derived growth factor (PDGF). Results: Compared with the model group, serum Hyp was significantly decreased after treatment with GFA-NAC · Mg, and the level of SOD in liver tissue was significantly increased and MDA significantly decreased. Liver fibrosis indicators TGF-β1, PDGF decreased in the tissue; GFA-NAC · Mg mesh staining showed that liver fibrosis improved. Conclusion: GFA-NAC · Mg has better experimental therapeutic effect on liver fibrosis rats, which may be achieved by inhibiting the expression of PDGF and TGF-β1.