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应用改良甲苯胺蓝(MTB)、阿尔新蓝-藏红O(AB/SO)、甲基绿-派洛宁(MG-P)、天青Ⅱ-伊红-瑞氏混合液等4种组化染色技术进行染色,并用小鼠抗人肥大细胞类胰蛋白酶单克隆抗体AAl通过ElivisionTM免疫组化染色方法,对虎纹蛙舌头肥大细胞进行染色。结果表明:虎纹蛙舌头中的肥大细胞,大小形态不一。虎纹蛙舌组织中分布有大量肥大细胞,主要分布于固有层的结缔组织中和舌头腺体之间,肌层也可见到大量的肥大细胞,并且有沿血管分布的特点。在用ElivisionTM免疫组化染色方法中,单克隆抗体AAl与虎纹蛙舌头组织中肥大细胞中的类胰蛋白酶有良好的交叉反应。类胰蛋白酶阳性细胞数量很少,分布于舌组织固有层和腺体之间。而人胃癌间质中可见多量类胰蛋白酶阳性细胞。
Four groups including MTB, AB / SO, MG-P, and Azure II-Eosin-Wrestler Staining technique was used to stain T. gonorrhoeae tongue mast cells by ElivisionTM immunohistochemical staining with mouse anti-human mast cell tryptase monoclonal antibody AAl. The results showed that the size and shape of mast cells in the tongue of tiger frogs varied. Tiger tongue frog tongue tissue distribution of a large number of mast cells, mainly in the connective tissue of the lamina propria and between the tongue gland, the myometrium can also see a large number of mast cells, and along the vascular characteristics. In the ElivisionTM immunohistochemical staining method, the monoclonal antibody AAl had a good cross-reactivity with tryptase in mast cells in tiger frog tongue tissue. Tryptase-positive cells in small quantities, located in lamina propria and glands between the tongue tissue. However, a large number of tryptase positive cells can be seen in human gastric cancer stroma.