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目的研究灯盏花素对梗阻性肾病大鼠肾间质及血管活性物质的影响。方法将SD大鼠随机分为假手术组、模型组和灯盏花素组。灯盏花素组给予灯盏花素200 mg.kg1灌胃,假手术组及模型组则给予0.5%羧甲基纤维素钠溶液灌胃,每天1次,连续7 d。HE及Masson染色观察大鼠肾组织病理改变,检测肾脏重量变化,血液及肾组织中转化生长因子β1(TGF-β1)及内皮素(ET-1)和一氧化氮(NO)的含量。结果模型组大鼠左侧肾脏肾间质可见大量炎性细胞浸润及充血,且重量与假手术组比较显著增加(P<0.01)。模型组大鼠血清中TGF-β1及NO、ET-1含量与假手术组相比存在明显差异(P<0.01)。两侧肾组织中TGF-β1及NO、ET-1含量分别与假手术组存在明显差异(P<0.01或P<0.001)。TGF-β1和ET-1含量升高而NO含量降低。而灯盏花素组大鼠肾间质损伤明显改善,左侧肾脏重量下降(P<0.05),血清中TGF-β1及NO、ET-1含量、肾组织中TGF-β1及NO、ET-1含量分别与模型组存在明显差异(P<0.05或P<0.01),TGF-β1及ET-1含量降低而NO升高。结论灯盏花素可通过下调血液及肾组织中TGF-β1和ET-1含量、上调NO浓度等作用减轻梗阻性肾病肾间质病变。
Objective To investigate the effects of breviscapine on renal interstitium and vasoactive substances in rats with obstructive nephropathy. Methods SD rats were randomly divided into sham operation group, model group and breviscapine group. Breviscapine group was given breviscapine 200 mg.kg1 gavage, sham operation group and model group were given 0.5% sodium carboxymethyl cellulose solution gavage once a day for 7 days. The pathological changes of kidney were observed by HE and Masson staining. The weight of kidney, the contents of TGF-β1, ET-1 and NO in blood and kidney were detected. Results A large amount of inflammatory cell infiltration and hyperemia were observed in the left renal interstitium in the model group, and the weight of the model group was significantly increased compared with the sham operation group (P <0.01). The levels of TGF-β1, NO and ET-1 in serum in model group were significantly different from those in sham operation group (P <0.01). The contents of TGF-β1, NO and ET-1 in bilateral renal tissues were significantly different from those in sham operation group (P <0.01 or P <0.001). TGF-β1 and ET-1 levels increased while NO levels decreased. In the breviscapine group, the renal interstitial injury was significantly improved, the left kidney weight was decreased (P <0.05), the content of TGF-β1, NO and ET-1 in the serum, TGF-β1 and NO, ET- (P <0.05 or P <0.01), while the content of TGF-β1 and ET-1 decreased but NO increased. Conclusion Breviscapine can alleviate the renal interstitial lesions of obstructive nephropathy by down-regulating the content of TGF-β1 and ET-1 in blood and kidney and up-regulating NO concentration.