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本工作通过测定大鼠血清、胰腺灌流液以及肤腺组织中胰岛素含量,观察生长抑素(SS)对链佐霉素(STZ)诱发的实验性糖尿病的作用。结果如下:皮下注射生理盐水后10min,再向腹腔注射链佐霉素(35mg/kg),24h 后大鼠血清胰岛素浓度明显降低。胰腺组织匀浆中的胰岛素含量也明显减少。如若在注射链佐霉素前10min 皮下注射生长抑素,则可有效地防止上述两项指标的改变,(NS+STZ)和(SS+STZ)两组之间具有显著差异。单独注射生长抑素,24h 后血清胰岛素及胰腺组织中胰岛素含量与正常对照无明显差异。用分离的大鼠胰腺作体外灌流,观察到:NS+STZ 组大鼠灌流胰腺对19.7mmol/L 的高浓度葡萄糖刺激无胰岛素释放反应,而 SS+STZ 组大鼠的胰腺对高浓度葡萄糖有反应性,刺激后出现胰岛素分泌峰。上述结果表明,SS(30μg/kg)预防性注射可以防止 STZ 引起的胰岛 B 细胞分泌功能的障碍。
In this study, the effects of somatostatin (SS) on streptozotocin (STZ) -induced experimental diabetes mellitus were observed by measuring the content of insulin in rat serum, pancreatic perfusate and dermal gland. The results were as follows: Subcutaneous injection of normal saline 10min, then to the intraperitoneal injection of streptozotocin (35mg / kg), 24h serum insulin was significantly lower. Insulin content in pancreatic homogenates also decreased significantly. If injected subcutaneously with somatostatin 10 min before injecting streptozotocin, the above two indexes can be effectively prevented. There is a significant difference between the two groups (NS + STZ) and (SS + STZ). Injecting somatostatin alone, serum insulin and pancreatic tissue after 24h insulin content and no significant difference between the normal control. The isolated rat pancreas was used for in vitro perfusion. It was observed that the perfused pancreas of NS + STZ group had no insulin-releasing response to high glucose of 19.7 mmol / L, while the pancreas of SS + STZ group had no effect on high glucose Reactivity, stimulated insulin secretion peak. The above results indicate that prophylactic injection of SS (30 μg / kg) can prevent the disorder of pancreatic B cell secretory function caused by STZ.