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目的:研究丹皮酚(Paeonol,Pae)对TNF-α诱导的人牙龈成纤维细胞(human gingival fibroblasts,HGF)黏附分子表达水平的影响。方法:使用50 ng/mL的肿瘤坏死因子(tumor necrosis factor,TNF)-α诱导刺激人牙龈成纤维细胞,后分别加入浓度为0、50、100、200 mmol/L的Pae孵育培养2、4、6天后,用Western blot法检测细胞间黏附分子(intercellular adhesion molecule,ICAM)-1以及血管细胞黏附分子(vascularcell adhesion molecule,VCAM)-1的蛋白表达水平,用逆转录多聚酶链反应(reverse transcription polymerase chain reaction,RT-PCR)检测黏附分子的mRNA表达水平。结果:TNF-α诱导的人牙龈成纤维细胞ICAM-1和VCAM-1的蛋白和mRNA表达水平均显著高于空白对照组(P<0.05);不同浓度的丹皮酚可显著降低TNF-α诱导的人牙龈成纤维细胞ICAM-1和VCAM-1的蛋白和mRNA表达(P<0.05),且随Pae浓度的升高逐渐减少。当Pae浓度达到100 mmol/L,蛋白和mRNA表达水平的抑制效果达到稳定程度(P>0.05),当Pae浓度达到200 mmol/L时,ICAM-1和VCAM-1 mRNA抑制效果达到诱导前的水平(P>0.05)。诱导时间的长短对蛋白和mRNA表达水平抑制效果没有显著影响(P>0.05)。结论:丹皮酚可显著降低TNF-α诱导的人牙龈成纤维细胞黏附分子表达水平。
Objective: To study the effect of paeonol on the expression of adhesion molecules induced by TNF-α in human gingival fibroblasts (HGF). Methods: Human gingival fibroblasts were induced by using 50 ng / mL tumor necrosis factor (TNF) -α, and then cultured in Pae at concentrations of 0, 50, 100, 200 mmol / After 6 days, the protein expression of intercellular adhesion molecule (ICAM) -1 and vascular cell adhesion molecule (VCAM) -1 was detected by Western blot. The expression of ICAM-1 and VCAM- polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of adhesion molecules. Results: The mRNA and protein expressions of ICAM-1 and VCAM-1 in human gingival fibroblasts induced by TNF-α were significantly higher than those in the blank control group (P <0.05). Different concentrations of paeonol could significantly decrease the expression of TNF-α The protein and mRNA expressions of ICAM-1 and VCAM-1 induced by human gingival fibroblasts were significantly decreased (P <0.05), and gradually decreased with the increase of Pae concentration. When Pae concentration reached 100 mmol / L, the inhibitory effect of protein and mRNA expression reached a steady level (P> 0.05). When Pae concentration reached 200 mmol / L, the inhibitory effect of ICAM-1 and VCAM- Level (P> 0.05). The induction time had no significant effect on the protein and mRNA expression level (P> 0.05). Conclusion: Paeonol can significantly reduce the TNF-α-induced human gingival fibroblast adhesion molecule expression levels.