论文部分内容阅读
目的:研究淋巴管形成及组织蛋白酶-D(Cath-D)的表达与喉癌颈淋巴结转移及临床病理因素的关系。方法:应用免疫组织化学SP法检测Cath-D在喉癌组织中的表达情况;使用肾小球足突细胞膜蛋白作为淋巴管内皮标记物,标记喉癌组织中淋巴管并测量淋巴管密度,以其癌旁正常组织作为对照。结果:①Cath-D的阳性表达率在正常黏膜、喉癌组织以及在不同病理分级、临床分期、有无颈淋巴结转移中有显著性差异;而在年龄及临床分型分组中无明显差异。②毛细淋巴管密度在癌周正常组织与癌旁以及在临床分期、临床分型、有无颈淋巴结转移中有显著性差异;而在年龄及病理分级分组中无明显差异。结论:①微淋巴管密度增高及Cath-D表达增高能促进喉癌的颈淋巴结转移;②微淋巴管密度增高和Cath-D表达增高在喉癌组织中有一定相关性,在喉癌淋巴结转移中具有协同作用。
Objective: To investigate the relationship between lymphangiogenesis and the expression of cathepsin-D (Cath-D) and cervical lymph node metastasis of laryngeal carcinoma and its clinicopathological factors. Methods: Immunohistochemical SP method was used to detect the expression of Cath-D in laryngeal squamous cell carcinoma. The glomerular foot process cell membrane protein was used as the endothelium marker of lymphatic vessel. The lymphatic vessels in laryngeal carcinoma were labeled and the density Its adjacent normal tissue as a control. Results: (1) The positive expression rate of Cath-D in normal mucosa, laryngeal cancer and in different pathological grade, clinical stage, with or without cervical lymph node metastasis were significantly different; but in age and clinical classification group no significant difference. ② lymphatic vessel density in cancer week normal tissue and adjacent cancer and in clinical stage, clinical type, with or without cervical lymph node metastasis were significantly different; but age and pathological grading group no significant difference. Conclusions: (1) The increase of lymphatic vessel density and the increase of Cath-D expression can promote the cervical lymph node metastasis of laryngeal carcinoma. (2) The increase of lymphatic vessel density and the increase of Cath-D expression in laryngeal carcinoma have some correlation. In synergy.